HPLC Separation of Albuterol and Amoxicillin

Primesep 100 separates albuterol and amoxicillin with baseline resolution by a combination of cation-exchange, hydrophobic, and polar interactions. The mass spec (LC/MS) compatible separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.  


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer TFA - 0.15%
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Albuterol,  Amoxicillin

Application Analytes: