Separation of Alginic Acid and Related Products

Alginate is used in various pharmaceutical preparations. Chemically, it is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. Alginic acid can be separated from benzoate, citric acid and saccharin by mixed-mode chromatography on Primesep C HPLC column. This method can be used to quantitate alginic acid, citric acid or saccharin in complex mixtures. Various detection technique can be used (UV, ELSD, LC/MS), based on mobile phase selection.

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Na2HPO4
Flow Rate 0.25 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Alginic acid, Citric acid, Benzoic acid, Saccharin
 

Application Analytes:

Alginic Acid
Benzoic Acid
Citric Acid
Saccharin