Separation of Diacid Hydrophobic and Ion Exchange Modes

Primesep B combines a hydrophobic, reversed-phase mechanism with ion exchange to separate the diacids, fumaric, benzoic, phthalic, naphthoic, and maleic acids. Changing the acetonitrile content of the mobile phase reverses the peak order for naphthoic and maleic acids. Primesep B combines reversed-phase and anion-exchange mechanism with a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and UV detection at 250 nm.


Column Primesep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Fumaric acid, Benzoic acid, Phthalic acid, Naphthoic acid, Maleic acid, )

Application Analytes:

Benzoic Acid
Dicarboxylic Acids
Fumaric Acid
Maleic Acid
Naphthoic Acid
Phthalic Acid