Separation of Epinephrine Norepinephrine

Primesep 200 separates the catecholamines, norepinephrine and epinephrine, less than 8 minutes. Norepinephrine and epinephrine are baseline resolved by a combination of reversed-phase, and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.

Condition

Column Primesep 200, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer  TFA - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Norepinephrine, Epinephrine
 

Application Analytes:

Epinephrine
Norepinephrine