Separation of Guanosine Diphosphate Mannose and Guanosine Diphosphate Fucose in Mixed-Mode Chromatography

Two nucleotides containing different sugar fragments were separated on Primesep SB mixed-mode HPLC column using LC/MS compatible conditions. Method can be used for analysis and isolation of guanosine diphosphate mannose and guanosine diphosphate fucose. Mechanism of retention is weak reversed-phase and strong anion-exchange. retention is controlled by buffer concentration and buffer pH. Both compounds exhibit strong interaction providing good retention and separation.

Condition

Column Primesep SB, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 2.9- 50 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Hydrophilic, Ionizable,  Nucleotide sugar
Analyzing Compounds Diphosphate Mannose, Guanosine Diphosphate Fucose
 

Application Analytes:

Guanosine Diphosphate Fucose
Guanosine Diphosphate Mannose