Separation of Mepiquat on Obelisc R Column

 chr_285.gif Mepiquat is a plant growth regulator that is monitored by EPA in water. It is a quaternary amine that is very polar in nature. Mepiquat and other hydrophilic amines are not retained on reversed-phase columns, and produce poor peak shape due to residual silanol interactions. Ion-pairing reagent is required to retain mepiquat. Ion-pairing reagents are not compatible with LC/MS detection and most environmental application require high sensitivity methods to determine very low level of contaminants. Obelisc R mixed-mode column can be used for EPA methods for determination of common pesticides, herbicides and insecticides where analysis of very polar ionic compounds is required.

Condition

Column Obelisc R,  4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C
 

Description

Class of Compounds Grow regulator,  Hydrophilic, Ionizable
Analyzing Compounds Mepiquat, Chloride


Application Analytes:

Chloride
Mepiquat