Separation of Quaternary Amines by Reverse-Phase Mechanism

Primesep B separates quaternary amines, such as t-butylamine with symmetrical peak shape by a combination of reversed-phase and ion-exclusion mechanisms. The embedded basic functional group on the stationary phase shields the underlying silanols to prevent peak tailing. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).


Column Primesep B,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Ions,  Hydrophilic, Ionizable, Quaternary amines
Analyzing Compounds t-butylamine

Application Analytes: