Separation of Small Lysine-based Peptide Oligomers

chr_302.gif Short-chain peptides are usually very polar compounds. If the peptide contains lysine moiety it also become very basic in nature. Analysis in traditional reversed-phase of HILIC mode can produce poor peak shape. The method was developed for lysine-based peptide on Primesep AP column in HILIC/cation-exclusion mode. Column can be used for analysis of small peptides with multiple basic groups. Compounds can be monitored by UV, LC/MS or ELSD.

Condition

Column Primesep AP, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV 215 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Lysine-based Peptide Oligomers
 

Application Analytes:

Lysine