Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC

Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.


Column Primesep 100,  4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Potassium,  Bromide, Ammonium

Application Analytes:

Ammonium Ion