Separation of Sodium, Tetramethylammonium and Tetrabutylammonium on Primesep 200 Column

chr_300.gif chr_301.gif Hydrophilic and hydrophobic quaternary amines, along with sodium ion, were separated by mixed-mode chromatography on a Primesep 200 column. Mechanism of retention for sodium and tetramethylammonium ions is cation exchange, while the tetrabutylammonium ion is retained by combination of reversed-phase and cation-exchange mechanisms. All three compounds are not UV-active and monitoring is done by ELSD/CAD.


Column Primesep 200,  4.6x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH4.0
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Ions,  Hydrophilic, Ionizable, Quaternary amines
Analyzing Compounds Sodium, Chloride, Tetramethylammonium,  Tetrabutylammonium

Application Analytes: