USP Analysis of Albuterol Using a Legacy L1 Column

  Application Notes: Albuterol is a well known drug used to treat bronchospasms. Albuterol come in many different forms, ranging from nebulizer to tablets. Albuterol is an important drug in combatting breathing problems. The USP methods for albuterol dictate that albuterol should not contain less than 98.5% and no more than 101% calculated on a dried basis. The USP HPLC method for the separation of albuterol was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP. Application Columns: Legacy L1 C18 HPLC column Application compounds: Albuterol Mobile phase: Water with 1% acetic acid and 5mM hexane sulfonic acid and MeOH (60:40) Detection technique: UV Reference: USP35: NF30

Condition

Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Water with 1% Acetic  and 5 mM   Hexane sulfonic acid/ MeOH  ( 60-40)
Buffer Acetic Acid,  Hexane sulfonic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Bronchodilator, Hydrophobic, Ionizable
Analyzing Compounds Albuterol
 

Application Analytes:

Albuterol