Application Notes: Dopamine is a naturally occurring neurotransmitter found in the brain. Dopamine is a well studied compound because dopamine is an important neurotransmitter known to regulate many functions from pain response to behavior disorders. According to USP methods, dopamine hydrochloride contains not less than 98% and not more than 102% dopamine hydrochloride calculated on the dried basis. The USP HPLC method for the separation of hydrocortisone was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Application Columns: Legacy L1 C18 HPLC column
Application compounds: Dopamine hydrochloride
Mobile phase: Water with 1% acetic and 5mM octanesulfonic acid/MeCN
Detection technique: UV
Reference: USP35: NF30
| Column | Legacy L1, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Water with 1% Acetic and 5 mM Octanesulfonic acid/ MeCN ( 87-13) |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Hydrophilic, Ionizable, Hormone, Acid |
| Analyzing Compounds | Dopamine |
Application Analytes:
Benzoic Acid
Dopamine