USP Method for the Analysis of Amoxicillin using the Legacy L1 Column


Application Notes: Amoxicillin is one of the most commonly prescribed antibiotics. It is often used for treating strep throat. According to USP methods, amoxicillin should not contain less than 900ug and no more than 1050ug of amoxicillin per mg.  The USP HPLC method for the separation of amitriptyline was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Amoxicillin

Mobile phase: MeCN/50 mM dibasic sodium phosphate

Detection technique: UV

Source: USP35: NF30 

Application Analytes: