USP Methods for the Analysis of Guaifenesin Using a Legacy L1 Column

  Application Notes: Guaifenesin is common, over the counter expectorant. Guaifenesin contain no less than 98 percent and not more than 102 percent of the labeled amount of guaifenesin calculated on a dried basis, according to the USP methods. the The USP HPLC method for the analysis of guaifenesin was developed on our Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Application Columns: Legacy L1 C18 HPLC column Application compounds: Guaifenesin, benzoic acid Mobile phase: MeOH/H2O/AcOH 40:60:1.5 Detection technique: UV Reference: USP 35- NF30  


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O/AcOH 40/60/1.5
Buffer NaH2PO4
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Antibiotics, Hydrophobic, Ionizable, Acid
Analyzing Compounds Guaifenesin, Benzoic acid

Application Analytes:

Benzoic Acid