Application Notes: Ibuprofen is the most commonly used analgesic. It is a vasoconstrictor and fever reducer. According to the USP methods, ibuprofen contains no less than 97% and no more than 103% ibuprofen based on a dried basis. The USP HPLC method for the separation of ibuprofen was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Application Columns: Legacy L1 C18 HPLC column
Application compounds: Ibuprofen and benzophenone
Mobile phase: .01M H3PO4/MeCN (55/45)
Detection technique: UV
Reference: USP35: NF30