Niacin (vitamin B3, nicotinic acid) is one of the essential vitamins. The water-soluble vitamin was analyzed by reversed-phase chromatography on the C18 reversed-phase HPLC column, Legacy L1. This column is classified according to USP as a L1-type of column. It can be used for the analysis of various drugs, vitamins, and chemicals according to United States Pharmacopeia.
| Column | Legacy L1, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Hexanesulfonate/MeOH/MeCN/AcOH –78/14/7/2 |
| Buffer | Hexanesulfonate |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Cholesterol medication, Vitamin B3, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamin B3, Niacin |
Application Analytes:
Vitamin B3 (Niacin)