USP Methods for the Analysis of Niacin with the Legacy L1 Column

Niacin (vitamin B3, nicotinic acid) is one of the essential vitamins. The water-soluble vitamin was analyzed by reversed-phase chromatography on the C18 reversed-phase HPLC column, Legacy L1. This column is classified according to USP as a L1-type of column. It can be used for the analysis of various drugs, vitamins, and chemicals according to United States Pharmacopeia.


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Hexanesulfonate/MeOH/MeCN/AcOH –78/14/7/2
Buffer Hexanesulfonate
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Drug, Cholesterol medication, Vitamin B3, Hydrophobic, Ionizable
Analyzing Compounds Vitamin B3, Niacin

Application Analytes:

Vitamin B3 (Niacin)