Application Notes: Procainamide is a common antiarrhythmic agent used to treat cardiac arrhythmias. According to USP methods procainamide hydrochloride contains not less than 98% and no more than 102% procainamide based on the dried basis. The USP HPLC method for the separation of procainamide and 4-aminobenzoic was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.
Reference: USP 35- NF30
| Column | Legacy L1, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O - 60/40% |
| Buffer | TEAPh pH 7.5 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Antiarrhythmic, Hydrophobic, Ionizable |
| Analyzing Compounds | Procainamide, 4-Aminobenzoic acid |
Application Analytes:
4-Aminobenzoic Acid
Procainamide
