By Column

Newcrom R1 (application examples)

Newcrom R1 stationary phase is generally stable at basic pH values.

Recommended pH is from 1.0 to 10.0. As an example, we performed a test where the column was operated at pH of 10.6 by injecting a model mixture of a neutral, basic, and acidic compounds.  This experiment took over 15 hours and after almost 200 injections no significant loss or changes in retention were observed.

However, despite the fact that this stationary phase is stable at high pH, it is recommended for column storage to use acidic mobile phase. A mobile phase composition with 0.1% phosphoric or formic acid in a 50/50 mixture of MeCN/H2O should be an ideal long and short term storage solution.

Newcrom R1 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm
Column Lengths: 250, 150, 100, 50, 25, 10 mm
Particles: 5 µm and 3 µm
Pores: 100 A

This column is available in two hardware versions traditional two ended and new SIELC hardware with only one end.

Newcrom



Legacy L1 (application examples)

Legacy L1 column meets the USP definition with a stationary phase that consists of octadecyl silane chemically bonded to porous silica or ceramic particles (1.5 to 10µm).

Legacy L1 columns are available in all standard dimensions 
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A



Promix AP (application examples)

Promix AP is a stationary phase for small and medium size peptides under 3 kDa with PI value below 7.0.

  • Reverse phase and ion-exchange mechanism of interaction.
  • Use acidic mobile phase pH from 2 to 5.5.
  • Use ion strength at least 20 mM.
  • 100Å and 300Å pore sizes are available.

Promix AP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 300 A, 100 A



Promix MP (application examples)

Promix MP is a stationary phase for medium size proteins and peptides from 1 to 10 kDa.

  • Broad pI value.
  • Reverse phase and ion-exclusion mechanism of interaction.
  • Use acidic mobile phase pH from 2 to 5.5.
  • Use ion strength at least 1 mM.
  • 300Å and 800Å pore sizes are available.

Promix MP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 800 A, 300 A



Promix SP (application examples)

Promix SP is a stationary phase for small peptides under 1 kDa with PI value below 6.0.

  • Reverse phase and ion-exchange mechanism of interaction.
  • Use acidic mobile phase pH from 2 to 5.5.
  • Use ion strength at least 20 mM.
  • 100Å pore size is available.

Promix SP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A



SHARC 1 (application examples)

SHARC 1 – Specific Hydrogen-bond Adsorption Resolution Chromatography

Sharc Chromatography Column

SHARC columns are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for  Specific Hydrogen-bond Adsorption Resolution Chromatography. The SHARC 1 column is the first of the SHARC series columns. 

 

SHARC1 columns are available in all standard dimensions 
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um, 3 um
Pores: 100 A



Obelisc N (application examples)

Obelisc N is a column with very polar characteristics that works well for polar and charged analytes.

  • Obelisc N offers both positively and negatively charged groups to interact with positively or negatively charged analytes.
  • Multiple separation modes (NP, HILIC, IE).
  • In HILIC mode, Obelisc N offers different selectivity form other HILIC or bare silica columns.
  • Mass spec, ELSD, preparative and low UV (<220 nm) compatible, low concentration buffers.

Obelisc N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Obelisc R (application examples)

Obelisc R is a reverse-phase analytical column that  can be used in traditional, reversed-phase type applications.

  • Due to the presence of ionic groups and a long hydrophobic chain, Obelisc R offers additional retention and tuning that is not available with traditional reversed-phase columns.
  • Multiple separation modes (RP, NP, IE).
  • Mass spec, ELSD, preparative and low UV (<220 nm) compatible, low concentration buffers.

Obelisc R columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep 100 (application examples)

Primesep 100 is a reverse-phase analytical column with embedded acidic ion-pairing groups

Primesep A - Acidic
This is the most versatile column of the entire Primesep family for separation of a broad range of compounds.

  • Improves retention of basic compounds by cation-exchange mechanism.
  • Separates acids by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.Primesep 100 HPLC column by Sielc

Primesep 100 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm, 3 µm, 2.7 µm (solid core)
Pores: 100 A



Primesep 200 (application examples)

Primesep 200 is a reverse-phase analytical column with embedded weak acidic ion-pairing groups.

Primesep A - Acidic

Improves retention of strong basic compounds by cation-exchange mechanism.

  • Retains neutral compounds by reverse-phase mechanism.
  • Provides an additional interaction with polar molecules offering separation of isomers and structurally-related compounds.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Reverse-phase analytical column Primesep 200

Primesep 200 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep 500 (application examples)

Primesep 500 is a reverse-phase analytical column with embedded acidic ion-pairing groups.

Primesep A - Acidic

  • Primesep 500 has an embedded carboxilic acid with a pKa of about 5.
  • Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
  • Separates acids by ion-exclusion mechanism at a mobile phase pH > 5.
  • Retains basic compounds, as any RP column, at pH < 5
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep 500 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep A (application examples)

Primesep A is a reverse-phase analytical column with embedded strong acidic ion-pairing groups.

Primesep A - Acidic

  • Improves retention of weak basic compounds by cation-exchange mechanism.
  • Separates acids by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep A columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep AB (application examples)

Primesep AB is a reverse-phase column with embedded acidic and basic ion-pairing groups.

  • Improves retention of strong acidic compounds and strong basic compounds by ion-exchange mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep AB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep B2 (application examples)

Primesep B2 is a reverse-phase analytical column with embedded basic ion-pairing groups.

Primesep B - Basic

  • Improves retention of acidic compounds by anion-exchange mechanism.
  • Separates bases by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5.5).

Primesep B2 columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep C (application examples)

Primesep C is a reverse-phase analytical column with embedded complex-forming groups.

Primesep C - Complex

  • Improves retention of positively charged compounds by forming a host-guest complex.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep C columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep D (application examples)

Primesep D is a reverse-phase analytical column with embedded basic ion-pairing groups specifically designed for direct plasma analysis.

Primesep B - Basic

  • Does not retain high-molecular weight plasma components.
  • Retains hydrophobic and acidic components.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 4).

Primesep D columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 100 A



Primesep N (application examples)

Primesep N is a normal-phase analytical column with embedded acidic groups.

  • Primesep N has embedded acidic groups with a pKa of about 5.
  • Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
  • Separates polar compounds by HILIC mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 300 A, 100 A



Primesep SB (application examples)

Primesep SB is a reverse-phase analytical column with strong embedded basic ion-pairing groups.

It contains a C18 carbon chain and acidic groups with pKa of 14.

 

Primesep B - Basic

  • Improves retention of acidic compounds by anion-exchange mechanism.
  • Separates bases by ion-exclusion mechanism.
  • Retains neutral compounds by reverse-phase mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5).

Primesep SB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm, 3 µm, 2.7 µm (solid core)
Pores: 100 A