HPLC Analysis on Xylose and Xylitol on Primesep S Column

Xylose is a monosaccharide which can be found in wood. Xylitol is a sugar-like, open chain polyalcohol. Xylitol and xylose were separated on the Primesep S HILIC/mixed-mode column. Both compounds are retained by a single HILIC mechanism. This method is compatible with ELSD, CAD, and RI detection and can be used for analysis of various monosaccharides in complex mixtures.

Condition

Column Primesep S, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Sugar, Hydrophilic, Ionizable
Analyzing Compounds  Xylose, Xylitol
 

Application Analytes:

Xylitol
Xylose