β‑Hydroxy-β-methylbutyric Acid (HMB)

CAS Number 625-08-1
Molecular Formula C5H10O3
Molecular Weight 118.13 g/mol
InChI Key AXFYFNCPONWUHW-UHFFFAOYSA-N
LogP -0.3
Synonyms
  • beta-Hydroxyisovaleric acid
  • 3-Hydroxy-3-methylbutanoic acid
  • 625-08-1
  • 3-Hydroxyisovaleric acid
  • 3-Hydroxy-3-methylbutyric acid
  • Hmb-d6
  • 3-OH-isovaleric acid
  • 3-hydroxy-isovaleric acid
  • 3-hydroxy-3-methyl-butanoic acid
  • Butanoic acid, 3-hydroxy-3-methyl-
  • beta-Hydroxy-beta-methylbutyrate
  • UNII-3F752311CD
  • B-hydroxyisovaleric acid
  • beta-hydroxy-beta-methylbutyric acid
  • MFCD00059081
  • 3-Hydroxyisovalerate
  • 3-hydroxy-3-methyl butyric acid
  • 3-hydroxy-3-methyl-Butyric acid
  • AXFYFNCPONWUHW-UHFFFAOYSA-N
  • 3-Hydroxy-3-methylbutyrate
  • 3F752311CD
  • 3-Hydroxy-3-Methyl butanoic Acid
  • 3-hydroxy-3-methyl-Butanoate
  • beta-Hydroxy-methylbutyrate
  • B-hydroxyisovalerate
  • Butyric acid, 3-hydroxy-3-methyl-
  • PubChem6288
  • AC1Q5VCY
  • B-hydroxy-b-methylbutyrate
  • AC1L2BP5
  • SCHEMBL95499
  • .beta.-Hydroxyisovaleric acid
  • Beta-(Hydroxyisovaleric Acid)
  • B-hydroxy-b-methylbutyric acid
  • beta.-Hydroxy-isovaleric acid
  • 3-methyl-3-hydroxybutyric acid
  • ACMC-1B512
  • CHEBI:37084
  • CTK7J1899
  • DTXSID20211535

Applications:


HPLC Separation of β‑Hydroxy-β-methylbutyric Acid, 2-Hydroxyisocaproic Acid and Leucine on Primesep 100 Column
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the essential dietary branched chain amino acid leucine.
 

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 5%, 3 min hold, Gradient MeCN 5-30% 12 min
Buffer H2SO4 - 0.05 % 3 min hold, GradientH2SO4 0.05-3% 12 min
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA)
Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA), Leucine
 

Application Analytes:

2-Hydroxyisocaproic Acid (HICA)
D-Leucine
Leucine
β‑Hydroxy-β-methylbutyric Acid (HMB)

HPLC Determination of β‑Hydroxy-β-methylbutyric Acid on Primesep B Column
HPLC Determination of β‑Hydroxy-β-methylbutyric Acid
Beta-Hydroxy beta-Methylbutyric acid (HMB) is used as an over-the-counter dietary supplement, which has been shown to increase lean body mass and muscle size. Another use for it is in to promote wound healing and to prevent the loss of muscle in individuals suffering from age-related muscle loss. HMB can be retained on a Primesep B mixed-mode column in in 3 minutes using low Acetonitrile (ACN) concentration in the mobile phase (10%) with 0.2% Phosphoric acid buffer. UV detection 200nm.
 

Condition

Column Primesep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H3PO4 - 0.2 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement
Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB)
     

Application Analytes:

β‑Hydroxy-β-methylbutyric Acid (HMB)

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid on Primesep 100 Colimn
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid_1182
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the essential dietary branched chain amino acid leucine.
 

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 5%
Buffer H2SO4 - 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA)
Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA)
 


Application Analytes:

2-Hydroxyisocaproic Acid (HICA)
β‑Hydroxy-β-methylbutyric Acid (HMB)