| CAS Number | 625-08-1 |
|---|---|
| Molecular Formula | C5H10O3 |
| Molecular Weight | 118.13 g/mol |
| InChI Key | AXFYFNCPONWUHW-UHFFFAOYSA-N |
| LogP | -0.3 |
| Synonyms |
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Applications:
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid, 2-Hydroxyisocaproic Acid and Leucine on Primesep 100 Column
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the essential dietary branched chain amino acid leucine.
| Column | Primesep 100, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN - 5%, 3 min hold, Gradient MeCN 5-30% 12 min |
| Buffer | H2SO4 - 0.05 % 3 min hold, GradientH2SO4 0.05-3% 12 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA) |
| Analyzing Compounds | β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA), Leucine |
Application Analytes:
2-Hydroxyisocaproic Acid (HICA)
D-Leucine
Leucine
β‑Hydroxy-β-methylbutyric Acid (HMB)
HPLC Determination of β‑Hydroxy-β-methylbutyric Acid on Primesep B Column
Beta-Hydroxy beta-Methylbutyric acid (HMB) is used as an over-the-counter dietary supplement, which has been shown to increase lean body mass and muscle size. Another use for it is in to promote wound healing and to prevent the loss of muscle in individuals suffering from age-related muscle loss. HMB can be retained on a Primesep B mixed-mode column in in 3 minutes using low Acetonitrile (ACN) concentration in the mobile phase (10%) with 0.2% Phosphoric acid buffer. UV detection 200nm.
| Column | Primesep B, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 10/90% |
| Buffer | H3PO4 - 0.2 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement |
| Analyzing Compounds | β‑Hydroxy-β-methylbutyric (HMB) |
Application Analytes:
β‑Hydroxy-β-methylbutyric Acid (HMB)
HPLC Separation of β‑Hydroxy-β-methylbutyric Acid and 2-Hydroxyisocaproic Acid on Primesep 100 Colimn
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the essential dietary branched chain amino acid leucine.
Condition
Column Primesep 100, 4.6x150 mm, 5 µm, 100A Mobile Phase MeCN - 5% Buffer H2SO4 - 0.05 % Flow Rate 1.0 ml/min Detection UV, 210 nm
Description
Class of Compounds Acid, Hydrophilic, Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA) Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA)
Application Analytes:
2-Hydroxyisocaproic Acid (HICA)
β‑Hydroxy-β-methylbutyric Acid (HMB)