2,3-Dihydroxybenzoic Acid

CAS Number 303-38-8
Molecular Formula C7H6O4
Molecular Weight 154.121 g/mol
InChI Key GLDQAMYCGOIJDV-UHFFFAOYSA-N
LogP 1.20
Synonyms
  • 2,3-Dihydroxybenzoic acid
  • Benzoic acid, 2,3-dihydroxy-
  • 303-38-8
  • o-Pyrocatechuic acid
  • 3-Hydroxysalicylic acid
  • 4-10-00-01414
  • Benzoic acid, 2,3-dihydroxy-
  • 1,2-Dihydroxybenzene-3-carboxylic acid
  • 2,3-Dihydroxybenzoesaure
  • acide 2,3-dihydroxybenzoique
  • acido 2,3-dihidroxibenzoico
  • Catecholcarboxylic acid
  • NSC 27435
  • Pyrocatechuic acid
  • BRN 2209117
  • EINECS 206-139-5
  • 2,3 DHB
  • 2,3-Dihydroxybenzoate
  • 2-Pyrocatechuate
  • 2-pyrocatechuic acid
  • 3-Hydroxysalicylate
  • Catecholcarboxylate
  • DHBA
  • DOBK
  • O-Pyrocatechuate
  • Pyrocatechuate
  • catechol-3-carboxylic acid

Applications:


Mixed-Mode Separation of Dihydroxybenzoic Acids



Primesep B offers better selectivity over traditional, reversed-phase C18 columns for separating regioisomers of aromatic dihydroxybenzoic acids (2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, 2,5-dihydroxybenzoic 3,4-dihydroxybenzoic, 3,5-dihydroxybenzoic acids) Primesep B combines reversed-phase and anion-exchange mechanism with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA).



Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid
Carboxylic Acids

HPLC Separation of Dihydroxybenzoic Acid


Separation of hydrophilic isomers is very difficult when only one mechanism of retention, like reversed-phase, is available. Isomers have very similar hydrophobic properties. In case of mixed-mode chromatography, small difference in hydrophobic and ionic properties allows to separate isomers. Isomers of dihydroxybenzoic acids are separated on a Primesep D column with good selectivity and peak shape. This method can be used for separation of other hydrophilic acidic compounds as well as hydrophobic basic compound.



Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid

Generic Screening Method for Complex Mixtures

Condition

Column Primesep 200, 4.6*150 mm 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 215 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethyl Paraben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil