4-Aminopyridine

4-Aminopyridine

CAS Number504-24-5
Molecular FormulaC5H6N2
Molecular Weight94.117
InChI KeyNUKYPUAOHBNCPY-UHFFFAOYSA-N
LogP0.3
Synonyms
  • Dalfampridine
  • 4-aminopyridine
  • pyridin-4-amine
  • Fampridine
  • 4-Pyridinamine
  • 4-Pyridylamine
  • Avitrol
  • p-Aminopyridine
  • 4-AP
  • Ampyra
  • gamma-Aminopyridine
  • Pyridine, 4-amino-
  • Amino-4 pyridine
  • Avitrol 200
  • Pimadin (free base)
  • Compound 1861
  • Phillips 1861
  • Fampridine-SR
  • FAMPYRA
  • Amaya
  • 4 Aminopyridine
  • Prc 1237
  • VMI 10-3
  • 4(1H)-Pyridinimine
  • Caswell No. 038
  • Neurelan
  • VMI-103
  • 4-amino-pyridine
  • UNII-BH3B64OKL9
  • 4- Aminopyridine
  • pyridin-4-ylamine
  • Amino-4-Pyridine
  • Mi-W-3
  • EL-970
  • NSC 15041
  • Ampyra (TN)
  • .gamma.-Aminopyridine
  • HSDB 6037
  • 4-AMINOPYRDINE

Applications:

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase

July 10, 2013

 

Condition

Column Sharc 1, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN – 97.5%
Buffer H3PO4 – 2.5%
Flow Rate 3.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds 2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a SHARC 1 HPLC Column

July 3, 2012

 

Application Notes: Pyridines and aminopyridines are hydrophilic basic compounds. Traditionally these compounds have been separated and analyzed by GC and HPLC. In the case of HPLC, reversed-phase chromatography with ion-pairing reagent is used along with alternative modes like HILIC for separation. Mixed-mode chromatography can also be used to successfully separate isomers of substituted pyridines. However, we developed a new mode of separation for these compounds with hydrogen bonding. Isomers of aminopyridine are separated based on hydrogen bonding interaction between analyte and stationary phase. Mobile phase utilizes combination of acetonitrile and methanol with additives. Retention time and selectivity are  sensitive to variations of mobile phase.  The order of elution changes depending on the amount of acetonitrile, methanol, formic acid and ammonium formate. This method and approach is compatible with  LC/MS and prep chromatography and can be used for separation of other pyridine based compounds and pyridine based isomers.

Application Columns: SHARC 1, 3.2×100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine

Detection Technique: UV, LC/MS  

Condition

Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Fa, AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds 2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Comparison of Obelisc R to C18 columns for the Separation of Aminopyridine Isomers

March 3, 2010


Three isomers of aminopyridine separate well by reversed-phase cation-exchange mixed-mode chromatography on an Obelisc R HPLC column. The presence of additional mechanism of interaction provides great selectivity for separation of closely related compounds. Elution of compounds can be monitored by UV, Evaporative Light-Scattering Detector (ELSD), Corona (CAD) or LC/MS. This method was validated at a pharmaceutical company. This HPLC method can be adopted as general approach for analysis of aminopyridine and other isomeric compounds.

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
2-Aminopyridine
3-Aminopyridine
4-Aminopyridine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.