Adenosine Diphosphate

CAS Number 18389-49-6
Molecular Formula C10H15N5O10P2
Molecular Weight 427.203 g/mol
LogP -4.6
  • Adenosine 5'-diphosphate
  • Adenosine diphosphate
  • ADP
  • adenosine-5'-diphosphate
  • adenosine pyrophosphate
  • Adenosine 5'-(trihydrogen diphosphate)
  • adenosine 5'-pyrophosphate
  • ADP (nucleotide)
  • adenosine-diphosphate
  • 5'-Adenylphosphoric acid
  • 5'-Adp
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphoric acid
  • Adenosine-5'-diphosphat
  • Adenosine 5'-diphosphoric acid
  • Adenosine diphosphoric acid
  • Ado-5'-P-P
  • H3adp
  • BRN 0067722
  • CHEBI:16761
  • EINECS 200-392-5
  • CHEMBL14830
  • 20398-34-9
  • AK-54732
  • 84412-16-8
  • adenosine-5-diphosphate


HPLC Separation of Adenosine Mono-, Di- and Triphosphate in Reversed-Phase Mixed-Mode with LC/MS Compatible Conditions
Adenosine mono-, di and triphosphate are hydrophilic nucleotides which serve as building blocks of DNA and RNA. Each molecule consists of phosphate or phosphate groups, adenine and sugar ribose. Molecules are hydrophilic and lack a retention mechanism on traditional reversed-phase column. Three nucleotides were retained and separated on Primesep B2 reversed-phase anion-exchange column. retention time is controlled by buffer concentration and buffer pH. ADP and ATP require higher concentration of buffer to facilitate elution. Method can be used for LC/MS analysis of different nucleotides in various sample matrices (biofluids, plasma, blood, urine). Other detection techniques can be used for analysis. Method is reliable and robust and can tolerate interference from sample matrix. Additional sample preparation might be required.  


Column Primesep B2, 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 2.9- 5-20 mM 3 min, 20-150 mM
Flow Rate 0.5 ml/min
Detection ELSD


Class of Compounds Nucleotide, Hydrophilic, Ionizable
Analyzing Compounds Adenosine Monophosphate, Adenosine Diphosphate, Adenosine Triphosphate

Application Analytes:

Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate

Separation of Nine Nucleotides by Mixed-Mode Chromatography
  Nucleotides are important biological molecules which serve as subunits of nucleic acids. They are composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Nucleotides cannot be retained by reverse-phase chromatography without an ion-pairing reagent due to their highly polar nature. Primesep SB is capable of retaining and separating nine nucleotides. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups.

Application Analytes:

Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate
Guanosine Diphosphate
Guanosine Monophosphate
Guanosine Triphosphate