Adenosine

CAS Number 58-61-7
Molecular Formula C10H13N5O4
Molecular Weight 267.245 g/mol
InChI Key OIRDTQYFTABQOQ-KQYNXXCUSA-N
LogP -1.05
Synonyms
  • Adenosine
  • 58-61-7
  • (-)-Adenosine
  • 9H-Purin-6-amine, 9-β-D-ribofuranosyl-
  • 9-β-D-Ribofuranosyl-9H-purin-6-amine
  • 9-β-D-Ribofuranosyladenine
  • Adenine riboside
  • Adenocard
  • Adenocor
  • Adenoscan
  • Adenosin
  • adenosina
  • Adrekar
  • Boniton
  • D-Adenosine
  • NSC 7652
  • Nucleocardyl
  • Riboadenosine
  • Sandesin
  • β-Adenosine
  • β-D-Adenosine
  • β-D-Ribofuranose, 1-(6-amino-9H-purin-9-yl)-1-deoxy-
  • β-D-Ribofuranoside, adenine-9
  • Caswell No. 010B
  • EINECS 200-389-9
  • 9H-Purin-6-amine, 9beta-D-ribofuranosyl-
  • beta-D-Ribofuranose, 1-(6-amino-9H-purin-9-yl)-1-deoxy-
  • beta-D-Ribofuranoside, adenine-9
  • NSC 627048
  • UNII-K72T3FS567
  • (2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
  • (2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
  • (2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-methylol-tetrahydrofuran-3,4-diol
  • (2R,3R,4S,5R)-2-(6-amino-9H-purin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
  • (2R,3R,4S,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
  • 1-(6-Amino-9H-purin-9-yl)-1-deoxy-beta-D-Ribofuranose
  • 1-(6-Amino-9H-purin-9-yl)-1-deoxy-beta-delta-Ribofuranose
  • 6-Amino-9-beta-D-ribofuranosyl-9H-purine
  • 6-Amino-9beta-D-ribofuranosyl-9H-purine
  • 6-Amino-9beta-delta-ribofuranosyl-9H-purine
  • 9-beta-D-Arabinofuranosyladenine
  • 9-beta-D-Ribofuranosidoadenine
  • 9-beta-D-Ribofuranosyl-9H-purin-6-amine
  • 9-beta-D-Ribofuranosyladenine
  • 9-beta-delta-Arabinofuranosyladenine
  • 9-beta-delta-Ribofuranosidoadenine
  • 9-beta-delta-Ribofuranosyl-9H-purin-6-amine
  • 9-beta-delta-Ribofuranosyladenine
  • 9beta-D-Ribofuranosyladenine
  • 9beta-D-ribofuranosyl-9H-Purin-6-amine
  • 9beta-delta-Ribofuranosyladenine
  • 9beta-delta-ribofuranosyl-9H-Purin-6-amine
  • Ade-Rib
  • Adenine Deoxyribonucleoside
  • Adenine nucleoside
  • Adenine-9beta-D-Ribofuranoside
  • Adenine-9beta-delta-Ribofuranoside
  • Adenyldeoxyriboside
  • Ado
  • Deoxyadenosine
  • Desoxyadenosine
  • Myocol
  • b-D-Adenosine
  • beta-Adenosine
  • beta-D-Adenosine
  • beta-delta-Adenosine
  • 46946-45-6
  • 46969-16-8

Applications:


HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N

Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm. 

Application Analytes:

Adenosine
Cytidine
Cytosine
Guanosine
Uracil
Uridine

HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method

 

Application Notes: Nucleosides are glycosylamines consisting of nucleobase linked to ribose or deoxyribose sugar and are building blocks for DNA and RNA. These compounds are very polar and contain groups available for hydrogen bonding interaction. Thymidine, uridine, adenosine, guanosine and cytidine were separated using a hydrogen-bonding method. There is a strong correlation between the retention time and mobile phase composition. The strength of hydrogen-bonding interaction increases as the number of hydroxyls in the analytes increase. Additionally the rder of elution for compounds depends on the ratio of the mobile phases: acetonitrile and methanol. Our method is compatible with LC/MS and preparative chromatography.

Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: Thymidine, uridine, adenosine, guanosine and cytidine




Application Analytes:

Adenosine
Cytidine
Guanosine
Thymidine
Uridine

HPLC Separation of Adenosine and Adenine Using the Hydrogen Bonding Method

 

 

Application Notes: Nucleosides are glycosylamines consisting of a nucleobase linked to a ribose or a deoxyribose sugar. Nucleoside are building blocks for DNA and RNA. These compounds are very polar in nature and contain groups available for hydrogen bonding interactions. Method for separation of adenine and adenosine were developed using a hydrogen-bonding method. There is a strong correlation between retention time for adenine/adenosine and the mobile phase composition, which consists of acetonitrile and methanol. Order of elution for compounds depends on the amount of acetonitrile and methanol.  Furthermore, ellution of adenine and adenosine can be reversed based on the composition of the mobile phase. Our method is compatible with LC/MS and preparative chromatography.

Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A. To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: Adenine and adenosinead

Detection Technique: UV, LC/MS



Application Analytes:

Adenine
Adenosine

HPLC Separation of Nucleosides and Deoxynucleosides

Th



Application Analytes:


Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Guanosine
Thymidine
Uridine

Separation of Model Compounds in Reversed-Phase and Mixed-Mode
   

Condition

Column Primesep 100, 3,2x50 mm, 2,7 µm, 100A
Mobile Phase Gradient  MeCN - 10-60%, 5 min
Buffer Gradient AmFm pH 3.5- 30 - 70 mM, 5 min
Flow Rate 1.2 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug,  Basic, Hydrophilic, Hydrophobic, Ionizable.
Analyzing Compounds Adenosine, 3,4-Difluroaniline, 4-Amino-2-chloropyridine, 5-Aminoindole, 4-Amino-3-chloropyridine, 2-Amino 5-methylthiadiazole, 4-Ethylaniline
 

Application Analytes:

2-Amino-5-Methylthiadiazole
2-Amino-5-methyl-thiazole
3,4-Difluoroaniline
4-Amino-2-Chloropyridine
4-Amino-3-Chloropyridine
4-Ethylaniline
5-Aminoindole
Adenosine