Alanine

CAS Number 56-41-7
Molecular Formula C3H7NO2
Molecular Weight 89.094 g/mol
InChI Key QNAYBMKLOCPYGJ-REOHCLBHSA-N
LogP -2.51
Synonyms
  • L-Alanine
  • L-Alanine
  • 56-41-7
  • Alanine, L-
  • (2S)-2-Aminopropanoic acid
  • (S)-(+)-Alanine
  • (S)-2-Aminopropanoic acid
  • (S)-Alanine
  • 1561: PN: US20060223088 SEQID: 1571 claimed protein
  • 2-Aminopropionic acid
  • Alanine
  • L-(+)-Alanine
  • L-2-Aminopropanoic acid
  • L-2-Aminopropionic acid
  • L-α-Alanine
  • L-α-Alanine
  • L-α-Aminopropionic acid
  • Lactamine
  • NSC 206315
  • Propanoic acid, 2-amino-, (S)-
  • α-Alanine
  • α-Aminopropionic acid
  • L-Alanin
  • L-alanina
  • ALANINE, L-
  • 6898-94-8
  • 115967-49-2
  • 170805-71-7
  • 759445-89-1
  • 787635-21-6
  • 1150316-17-8

Applications:


Amino Acids Analysis in Acid Gradient Condition



11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).



Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Application Analytes:

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

HPLC Separation of Lysine and Arginine from Other Amino Acids

 

Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge.

Application Columns: Primesep C
Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
Detection technique: UV, LC/MS, ELSD/CAD



Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine