Applications:
Separation of Alginic Acid and Related Products

Alginate is used in various pharmaceutical preparations. Chemically, it is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. Alginic acid can be separated from benzoate, citric acid and saccharin by mixed-mode chromatography on Primesep C HPLC column. This method can be used to quantitate alginic acid, citric acid or saccharin in complex mixtures. Various detection technique can be used (UV, ELSD, LC/MS), based on mobile phase selection.
Condition
Column |
Primesep C, 4.6x150 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
Na2HPO4 |
Flow Rate |
0.25 ml/min |
Detection |
UV, 210 nm |
Description
Class of Compounds
|
Acid, Hydrophilic, Ionizable |
Analyzing Compounds |
Alginic acid, Citric acid, Benzoic acid, Saccharin |
Alginic AcidBenzoic AcidCitric AcidSaccharin
HPLC Analysis of Alginic Acid in Heartburn Tablets

Alginic acid is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. The monomers can appear in homopolymeric blocks of consecutive G-residues (G-blocks), consecutive M-residues (M-blocks), alternating M and G-residues (MG-blocks), or randomly organized blocks. Molecular weight varies from 10,000 to 600,000. Analysis is a challenging task due to a wide distribution of molecular weights. Alginic acid analyzed in ion-exclusion and size exclusion mode on Primesep C mixed-mode cation exchange column. Alginic acid elutes before void as a single peak with good symmetry. Method can be used for quantitation of alginic acid in various formulation. Low UV or ELSD can be employed.
Alginic Acid