Bufferless Ion Separation (BLIS™) Chromatography of Aspartame: Organic Modifier Effect
An organic modifier effect of changing acetonitrile percentage the mobile phase is demonstrated on a Primesep C HPLC column in a bufferless ion (BLIS™) separation. With decreasing percent acetonitrile, the artificial sweetener, Aspartame, first deceases in retention time and then increases. The changing mobile phase composition changes the retention mechanism from hydrophobic and BLIS™interactions, to BLIS™ alone , to BLIS™ and HILIC (hydrophilic liquid chromatography) interactions. Aspartame is composed of the two amino acids, aspartic acid and phenylalanine as the ethyl ester. Mobile phases composed of water and acetonitrile (MeCN, ACN,) and ultraviolet (UV) detection was used.
HPLC Separation of Amino Acids on Obelisc R Column
Closely related compounds like amino acids can be separated on an Obelisc R column by various buffers depending on the amount of baseline separation required. By choosing different buffers, the separation between compounds can be adjusted based on application needs, especially those that require low organic concentration in the mobile phase. UV detection at 250nm.
||Obelisc R, 4.6x250 mm, 5 µm, 100A
||MeCN/H2O - 5/95%
||UV, 250 nm
|Class of Compounds
||Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid