Aspartic Acid

CAS Number 56-84-8
Molecular Formula C4H7NO4
Molecular Weight 133.103 g/mol
InChI Key CKLJMWTZIZZHCS-REOHCLBHSA-N
LogP -3.89
Synonyms
  • L-Aspartic acid
  • 56-84-8
  • (S)-2-Aminobutanedioic acid
  • L-Asparagic acid
  • (+)-Aspartic acid
  • (S)-Aminobutanedioic acid
  • (S)-Aspartic acid
  • 2-Aminobutanedioic acid
  • Acide aspartique
  • acido aspartico
  • Asparagic acid
  • Asparaginic acid
  • Asparaginsaure
  • aspartic acid
  • Aspartic acid, L-
  • Butanedioic acid, amino-, (S)-
  • H-Asp-OH
  • L-(+)-Aspartic acid
  • L-Aminosuccinic acid
  • L-Asparaginic acid
  • NSC 3973
  • NSC 79553
  • L-2-Aminobutanedioic acid
  • Aminosuccinic acid
  • L-Asparaginsyra
  • Aspartate, L-
  • Aspatofort
  • BRN 1723530
  • EINECS 200-291-6
  • Acidum asparticum
  • L(+)-Aminobernsteinsaeure
  • L-Aspartinsaeure
  • Asparaginsaeure
  • UNII-30KYC7MIAI
  • (+)-Aspartate
  • (2S)-2-aminobutanedioic acid
  • (2S)-2-aminosuccinic acid
  • (2S)-Aspartate
  • (2S)-Aspartic acid
  • (L)-Aspartate
  • (L)-Aspartic acid
  • (R)-2-aminosuccinate
  • (S)-(+)-Aspartate
  • (S)-(+)-Aspartic acid
  • (S)-2-aminosuccinate
  • (S)-2-aminosuccinic acid
  • (S)-Aminobutanedioate
  • (S)-Aspartate
  • (S)-amino-Butanedioate
  • (S)-amino-Butanedioic acid
  • 2-Amino-3-methylsuccinate
  • 2-Amino-3-methylsuccinic acid
  • 2-Aminosuccinate
  • Aminosuccinate
  • Asparagate
  • Asparaginate
  • Asparatate
  • Aspartate
  • L-(+)-Aspartate
  • L-Aminosuccinate
  • L-Asparagate
  • L-Asparaginate
  • L-Asparaginsaeure
  • L-Aspartate
  • alpha-Aminosuccinate
  • alpha-Aminosuccinic acid
  • 6899-03-2
  • 181119-33-5

Applications:


Amino Acids Analysis in Acid Gradient Condition
11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Gaspartic acid, Glutamic acid, Alanine, Valine, Methionine, Isoleucine, Cysteine, Phenylalanine, Histidine, Lysine, Arginine
 

Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

HPLC Separation of Polar Compounds

The separation of amino acids, the building blocks of proteins, can be challenging to separate on a reverse-phase column due to their high polarity. Using a mixed-mode HPLC column, allows the separation of amino acids by cation-exchange and ion-exclusion mechanisms as well as hydrophobicity. Fine tuning of separation can be achieved with changes in organic concentration of the mobile phase as well as choice of buffer and pH.

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AcOH, AmFm
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Aspartic acid, Glutaric acid, Glycine, Hydroxytriptophan, GABA, Norepinephrine, Dopamine
 

Application Analytes:


Aspartic Acid
Dopamine
Glutamic Acid
Norepinephrine
gamma-Aminobutyric Acid (GABA)

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Application Analytes:

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

Effect of both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids


In mixed-mode HILIC chromatography, selectivity of separation can be adjusted by amount of acetonitrile, amount of buffer and buffer pH. Buffer concentration and pH will affect retention of ionizable compounds to a different degree. Retention of neutral compounds can be adjusted by the amount of acetonitrile. Carboxylic acid, three amino acids and two sugars are separated by combination of HILIC and ion-exchange mechanisms. Compounds can be monitored by ELSD, Corona (CAD), LC/MS or low UV. UV-transparent mobile phase /buffer is required for UV monitoring of this mixed-mode separation. This HPLC method can be adopted as general approach for analysis of sugars, amino acids and carboxylic acids.



Application Analytes:

Aspartic Acid
Phenylalanine
Succinic Acid

HPLC Separation of Aspartic Acid and Asparagine on Obelisc R Column


Two amino acids, aspartic acid and asparagine, are separated on an Obelisc R column by combination of reverse-phase and ion-exchange mechanism. Method can be used for analysis of these and other underivatized amino acids by HPLC with UV, ELSD, CAD and LC/MS detection.



Application Analytes:

Asparagine
Aspartic Acid

HPLC Separation of Lysine and Arginine from Other Amino Acids
  Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge. Application Columns: Primesep C Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 15/85%
Buffer AmFm pH 5.0- 15 mM
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
 

Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

HPLC Separation of Mixture of Non-Essential Amino Acids
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

Amino Acids
Aspartic Acid
D-Alanine
DL-Alanine
GLU (L-Glutamic acid)
Glutamic Acid
Serine