Aspartic Acid

CAS Number	56-84-8
Molecular Formula	C4H7NO4
Molecular Weight	133.103 g/mol
InChI Key	CKLJMWTZIZZHCS-REOHCLBHSA-N
LogP	-3.89
Synonyms	L-Aspartic acid 56-84-8 (S)-2-Aminobutanedioic acid L-Asparagic acid (+)-Aspartic acid (S)-Aminobutanedioic acid (S)-Aspartic acid 2-Aminobutanedioic acid Acide aspartique acido aspartico Asparagic acid Asparaginic acid Asparaginsaure aspartic acid Aspartic acid, L- Butanedioic acid, amino-, (S)- H-Asp-OH L-(+)-Aspartic acid L-Aminosuccinic acid L-Asparaginic acid NSC 3973 NSC 79553 L-2-Aminobutanedioic acid Aminosuccinic acid L-Asparaginsyra Aspartate, L- Aspatofort BRN 1723530 EINECS 200-291-6 Acidum asparticum L(+)-Aminobernsteinsaeure L-Aspartinsaeure Asparaginsaeure UNII-30KYC7MIAI (+)-Aspartate (2S)-2-aminobutanedioic acid (2S)-2-aminosuccinic acid (2S)-Aspartate (2S)-Aspartic acid (L)-Aspartate (L)-Aspartic acid (R)-2-aminosuccinate (S)-(+)-Aspartate (S)-(+)-Aspartic acid (S)-2-aminosuccinate (S)-2-aminosuccinic acid (S)-Aminobutanedioate (S)-Aspartate (S)-amino-Butanedioate (S)-amino-Butanedioic acid 2-Amino-3-methylsuccinate 2-Amino-3-methylsuccinic acid 2-Aminosuccinate Aminosuccinate Asparagate Asparaginate Asparatate Aspartate L-(+)-Aspartate L-Aminosuccinate L-Asparagate L-Asparaginate L-Asparaginsaeure L-Aspartate alpha-Aminosuccinate alpha-Aminosuccinic acid 6899-03-2 181119-33-5

Applications:

Amino Acids Analysis in Acid Gradient Condition

11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

HPLC Separation of Polar Compounds

The separation of amino acids, the building blocks of proteins, can be challenging to separate on a reverse-phase column due to their high polarity. Using a mixed-mode HPLC column, allows the separation of amino acids by cation-exchange and ion-exclusion mechanisms as well as hydrophobicity. Fine tuning of separation can be achieved with changes in organic concentration of the mobile phase as well as choice of buffer and pH.

Aspartic Acid
Dopamine
Glutamic Acid
Norepinephrine
gamma-Aminobutyric Acid (GABA)

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase. Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

Effect of both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids

In mixed-mode HILIC chromatography, selectivity of separation can be adjusted by amount of acetonitrile, amount of buffer and buffer pH. Buffer concentration and pH will affect retention of ionizable compounds to a different degree. Retention of neutral compounds can be adjusted by the amount of acetonitrile. Carboxylic acid, three amino acids and two sugars are separated by combination of HILIC and ion-exchange mechanisms. Compounds can be monitored by ELSD, Corona (CAD), LC/MS or low UV. UV-transparent mobile phase /buffer is required for UV monitoring of this mixed-mode separation. This HPLC method can be adopted as general approach for analysis of sugars, amino acids and carboxylic acids.

Aspartic Acid
Phenylalanine
Succinic Acid

HPLC Separation of Aspartic Acid and Asparagine on Obelisc R Column

Two amino acids, aspartic acid and asparagine, are separated on an Obelisc R column by combination of reverse-phase and ion-exchange mechanism. Method can be used for analysis of these and other underivatized amino acids by HPLC with UV, ELSD, CAD and LC/MS detection.

Asparagine
Aspartic Acid

HPLC Separation of Lysine and Arginine from Other Amino Acids

Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge.

Application Columns: Primesep C
Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
Detection technique: UV, LC/MS, ELSD/CAD

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

HPLC Separation of Mixture of Non-Essential Amino Acids

Amino Acids
Aspartic Acid
D-Alanine
DL-Alanine
GLU (L-Glutamic acid)
Glutamic Acid
Serine