Benzalkonium Chloride

Applications:


HPLC Separation of Surfactants

Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.

Condition

Column Primesep D,  4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210nm
 

Description

Class of Compounds Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100, Cetylpyridinium Chloride


Application Analytes:

Benzalkonium Chloride
Cetylpyridinium Chloride
Sodium
Triton X100

HPLC Separation of Benzalkonium Chloride BCT 8358 on Primesep D column
chr_289.gif Benzalkonium chloride is a mixture of alkylbenzyldimethylammonium chlorides of various even-numbered alkyl chain lengths. It is used as a surfactant. Hydrophobic quaternary amines, like BTC, produce poor peak shape in reversed-phase chromatography due to interaction of quaternary amines with residual silanols. Ideal peak shape can be achieved on reversed-phase anion-exchange columns like Primesep D. Primesep D has a basic group on the surface of silica gel that shields silanols from interacting with quaternary, tertiary, secondary and primary amines. This allows to achieve symmetrical peaks for hydrophobic basic analytes. Compounds need to be hydrophobic enough to get compensation for repulsion effect coming from stationary phase. BTC can be monitored by ELSD and UV. Primesep D will also retain acidic counter-ions of quaternary amines, so simultaneous analysis of hydrophobic basic and hydrophilic basic compounds is possible.

Condition

Column Primesep D, 4,6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection 210
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Description

Class of Compounds  Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Benzalkonium Chloride BCT 8358


Application Analytes:

Benzalkonium Chloride