Benzalkonium Chloride

Applications:


HPLC Separation of Surfactants

Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.  

Application Analytes:

Benzalkonium Chloride
Cetylpyridinium Chloride
Sodium
Triton X100

HPLC Separation of Benzalkonium Chloride BCT 8358 on Primesep D column

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Benzalkonium chloride is a mixture of alkylbenzyldimethylammonium chlorides of various even-numbered alkyl chain lengths. It is used as surfactant. Hydrophobic quaternary amines, like BTC, produce poor peak shape in reversed-phase chromatography due to interaction of quaternary amines with residual silanols. Ideal peak shape can be achieved on reversed-phase anion-exchange columns like Primesep D. Primesep D has a basic group on the surface of silica gel that shields silanols from interacting with quaternary, tertiary, secondary and primary amines. This allows to achieve symmetrical peaks for hydrophobic basic analytes. Compounds need to be hydrophobic enough to get compensation for repulsion effect coming from stationary phase. BTC can be monitored by ELSD and UV. Primesep D will also retain acidic counter-ions of quaternary amines, so simultaneous analysis of hydrophobic basic and hydrophilic basic compounds is possible.



Application Analytes:

Benzalkonium Chloride