Calcium

CAS Number7440-70-2
Molecular FormulaCa
Molecular Weight40.078
InChI KeyFAQLAUHZSGTTLN-UHFFFAOYSA-N
Synonyms
  • Calcium
  • 7440-70-2
  • Atomic calcium
  • Blood-coagulation factor IV
  • Calcium atom
  • CALCIUM CRYSTALS
  • Calcium element
  • CALCIUM, ANHYDROUS
  • UN 1401
  • UN 1855
  • EINECS 231-179-5
  • Elemental calcium
  • UNII-SY7Q814VUP
  • Calcium, elemental
  • 20Ca
  • Ca
  • Kalzium
  • calcio
  • Ca(0)
  • Can
  • calcium(0)
  • 8047-59-4

Applications:

HPLC Analysis of Calcium Ions in Maple Syrup

July 10, 2012

 

Application Notes: maple syrup primarily consists of sucrose and other sugars, but also has small amounts of amino acids (arginine, threonine, proline), organic acids (malic and fumaric), vitamins (niacin and riboflavin) and traces of minerals (Na, K, Ca, Mn, Mg, Zn ions). One analysis performed on maple syrup is calcium ion determination. A Primesep 100 column was used in cation-exchange mode to isolate calcium ion from other components of the maple syrup. This method can be used for quantification of calcium ion by ELSD/CAD. Limit of detection depends on the type of ELSD/CAD detector used but usually reaches 20 ppm

Application Columns: Primesep 100
Application compounds: calcium ions, Maple Syrup
Detection technique: LC/MS, ELSD/CAD

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Ions, Hydrophilic, Ionizable
Analyzing Compounds Maple Syrup,  Calcium

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Calcium
Organic Acids
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions

July 8, 2011

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Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes.

Condition

Column Primesep N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD 50C

 

Description

Class of Compounds
Ions, Hydrophilic, Ionizable
Analyzing Compounds Potassium, Sodium, Calcium, Glucose

 

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Calcium
Potassium
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column

October 4, 2005

Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.

ColumnPrimesep 100, 4.6×250 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 70/30%
BufferTFA- 0.4%
Flow Rate1.0 ml/min
DetectionELSD 50C
Class of Compounds
Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing CompoundsSodium, Magnesium, Calcium, Lithium, Barium

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Barium
Bromide
Calcium
Lithium
Magnesium
Potassium
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.