Cannabidiol (CBD)

Cannabidiol

CAS Number 3556-78-3
Molecular Formula C21H30O2
Molecular Weight 314.469 g/mol
InChI Key QHMBSVQNZZTUGM-ZWKOTPCHSA-N
LogP 6.5
Synonyms
  • cannabidiol
  • (-)-Cannabidiol
  • 13956-29-1
  • (-)-trans-Cannabidiol
  • UNII-19GBJ60SN5
  • (-)-trans-2-p-Mentha-1,8-dien-3-yl-5-pentylresorcinol
  • delta1(2)-trans-Cannabidiol
  • (-)-CBD
  • GWP42003-P
  • CBD
  • 19GBJ60SN5
  • CHEMBL190461
  • CHEBI:69478
  • QHMBSVQNZZTUGM-ZWKOTPCHSA-N
  • Epidiolex
  • 1,3-Benzenediol, 2-(3-methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl)-5-pentyl-, (1R-trans)-
  • 2-[(1R,6R)-3-methyl-6-prop-1-en-2-ylcyclohex-2-en-1-yl]-5-pentylbenzene-1,3-diol
  • (1'R,2'R)-5'-methyl-4-pentyl-2'-(prop-1-en-2-yl)-1',2',3',4'-tetrahydrobiphenyl-2,6-diol
  • Resorcinol, 2-p-mentha-1,8-dien-3-yl-5-pentyl-, (-)-(E)-
  • Cannabidiol [USAN]

Applications:


Fast Separation of Cannabinoids on Mixed-Mode HPLC Column Cannsep B

With legalization of marijuana in much of the United States, more and more attention is paid to developing quick, robust and reliable method for quantitation of cannabinoids in marijuana plants and related edible products. The main challenge lies in sample preparation and quantitative analysis of four major cannabinoids: cannabidiol, cannabinol, tetrahydrocannabinol and tetrahydrocannabinolic acid. All compounds are hydrophobic with only THCA (THC-A) and cannabidiolic acid (CBDA) being an acid. Hydrophobic interaction is the main interaction in the separation of cannabinoids on reverse phase columns. On our mixed mode columns acidic molecules retained by ion-exchange mechanism  in addition to hydrophobic. Method can be used for analysis and prep separation of cannabinoids in marijuana plants, seeds and other cannabis-based products. Extraction of cannabinoids is the main procedure in sample preparation. Extraction can be done with organic solvents like chloroform, methanol, ethanol, acetonitrile.  Cannabinoids can be monitored by UV and LC/MS. Mixed-mode columns containing polar embedded groups provide different selectivity than regular reversed-phase columns.

 

Condition

Column Cannsep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 40-60% , 20 min
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)
 

Application Analytes:

Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)

HPLC Separation of Ten Cannabinoids on Cannsep A Column

Condition

Column Cannsep A, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 50-70% , 20 min
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)
 

Application Analytes:

Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)

HPLC Separation of Ten Cannabinoids on Cannsep B Column

Condition

Column Cannsep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 50-70% , 25 min
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)
 

Application Analytes:

Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)

HPLC Separation of Ten Cannabinoids on Cannsep C Column

Condition

Column Cannsep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 60-80% , 20 min
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)
 

Application Analytes:

Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)

HPLC Determination of THC in THC Infused Gummy Bear on Cannsep C Column

Condition

Column Cannsep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 60-80%, 25 min, 5 min hold
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)
 

Application Analytes:

Cannabidiol (CBD)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)

HPLC Determination of CBD, CBN, Δ9-THC, Δ8-THC on Cannsep C Column
HPLC.cloud View on hplc.cloud Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Cannsep C, 3.2x150 mm, 3 µm, 100A
Mobile Phase MeOH -80%,
Buffer H2SO4 - 0.2%
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC
 

Application Analytes:

Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (THC)
Delta-9-Tetrahydrocannabinol (THC)

HPLC Determination of Δ8-THC, CBD, Δ9-THC, CBN, on Primesep N Column
HPLC.cloud View on hplc.cloud Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.

Condition

Column Primesep N , 3.2x150 mm, 3 µm, 100A
Mobile Phase Hexane/Ethyl Acetate - 97/3%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 275 nm
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Delta -8-Tetrahydrocannabinol, Δ8-THC, Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol, Δ9-THC, Cannabinol (CBN)
 

Application Analytes:

Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (THC)
Delta-9-Tetrahydrocannabinol (THC)