Carboxylic Acids

Carboxylic Acids

Applications:


Separation of Aromatic Carboxylic Acids by Mixed-Mode HPLC
This application on Primesep B demonstrates the easy tuning of methods developed on Primesep columns. Aromatic carboxylic acids are easily separated and retention times can be shifted by simply changing the mobile phase modifier. Switching between formic, trifluoroacetic acid, phosphoric, and perchloric acids in a constant ratio of acetonitrile and water changes elution times, which is not possible on reversed-phase C18 columns. This effect is due to both the change in mobile phase pH and the counter ion that interacts with the basic functional group on the column. Mass spec compatible mobile phases of water, acetonitrile (MeCN, ACN) and, formic or trifluoracetic acid (TFA) can be chosen for LC/MS applications.

Condition

Column Primesep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Carboxylic Acids
 

Application Analytes:

Aromatic Acids
Carboxylic Acids

Mixed-Mode Separation of Dihydroxybenzoic Acids
Primesep B offers better selectivity over traditional, reversed-phase C18 columns for separating regioisomers of aromatic dihydroxybenzoic acids (2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, 2,5-dihydroxybenzoic 3,4-dihydroxybenzoic, 3,5-dihydroxybenzoic acids) Primesep B combines reversed-phase and anion-exchange mechanism with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA).

Condition

Column Primesep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds 3.4-dihydroxybenzoic acid, 3.5-dihydroxybenzoic acid,2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid
Carboxylic Acids

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