Creatinine

CAS Number 60-27-5
Molecular Formula C4H7N3O
Molecular Weight 113.120 g/mol
InChI Key DDRJAANPRJIHGJ-UHFFFAOYSA-N
LogP -1.76
Synonyms
  • Creatinine
  • 2-Imino-1-methylimidazolidin-4-one
  • 4-Imidazolidinone, 2-imino-1-methyl-
  • 60-27-5
  • 4H-Imidazol-4-one, 2-amino-1,5-dihydro-1-methyl-
  • 1-Methylglycocyamidine
  • 1-Methylhydantoin-2-imide
  • 2-Amino-1,5-dihydro-1-methyl-4H-imidazol-4-one
  • 2-Amino-1-methyl-1,5-dihydroimidazol-4-one
  • 2-Amino-1-methylimidazolin-4-one
  • 2-Imino-1-methylimidazolidin-4-on
  • 2-Imino-1-methylimidazolidin-4-one
  • 2-imino-1-methylimidazolidine-4-one
  • 2-imino-1-metilimidazolidin-4-ona
  • KREATININE
  • NSC 13123
  • TEGO Cosmo C 250
  • Creatinine,heated
  • EINECS 200-466-7
  • UNII-AYI8EX34EU
  • 2-amino-1-methyl-5H-imidazol-4-one
  • Creatine anhydride
  • Kreatinin
  • creatinina
  • 15231-31-9
  • 45514-66-7
  • 82016-55-5

Applications:


HPLC Separation of Neurotransmitters
The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead, a combination of ion-exchange and reversed-phase interactions were used.

Condition

Column Primesep 200, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer TFA - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds dl-DOPA, Creatinine, Hydroxytryptophan (5-HTP), Serotonin
 

Application Analytes:

Creatinine
DOPA (3,4-dihydroxy-L-phenylalanine)
Hydroxytryptophan
Serotonin

HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions
Creatine, creatinine, and histidine are all important amino acids in the human body. All of these can be separated by liquid chromatography. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Condition

Column Primesep 200, 3.0x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic Acid - 0.1%
Flow Rate 0.5 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Creatine, Creatinine, Histidine
 

Application Analytes:

Creatine
Creatinine
Histidine

HPLC Separation of Creatine, Creatinine and Histidine
Creatine is produced by the body and plays a major role in recycling ATP. It also stabilizes pH in certain tissues. Creatine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 1/90%
Buffer TFA -0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Creatine, Creatinine, Histidine
 

Application Analytes:

Creatine
Creatinine
Histidine