Cytidine Monophosphate

CAS Number 30811-80-4
Molecular Formula C9H14N3O8P
Molecular Weight 323.198 g/mol
LogP -3.4
  • Cytidine 5'-monophosphate
  • cytidylic acid
  • Cytidine monophosphate
  • Cytidine-5'-monophosphate
  • 5'-CMP
  • cytidylate
  • Cytidine 5'-phosphate
  • CMP (nucleotide)
  • Cytidine 5'-phosphoric acid
  • Poly(rC)
  • Cytidine 5'-monophosphoric acid
  • Poly(cytidylic acid)
  • Polyribocytidylic acid
  • Cytidine 5'-(dihydrogenphosphate)
  • 30811-80-4
  • cytidine-P
  • cytidine-phosphate
  • Cytidine 3'-(dihydrogen phosphate)
  • Polyribonucleotide complex C
  • 1beta-Ribofuranosylcytosine 5'-phosphate, d-
  • C-5'-P
  • CMP
  • 5'-Cytidylic acid(homopolymer)
  • ((2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate
  • POLY C
  • CHEBI:17361
  • EINECS 200-556-6
  • Cytidine 5 -monophosphate


HPLC Separation of Cytidines

Cytidine phosphates are nucleotides. Nucleotides contain a nucleobase, five-carbon sugar and one or three phosphate groups. All three cytidines are very polar and cannot be retained by reversed-phase chromatography without a ion-pairing reagent. Mixed-mode Primesep SB column easily allows to retain and separate mono-, di-, and tri-phospates by anion-exchange mechanism. Other nucleotides can be separated as well by this approach. Nucleotides in biofluids can be analyzed directly on this column without any significant sample preparation. Most of the proteins from biological fluids will be repelled from the surface of silica gel. Method is compatible with UV, ELSD, CAD and LC/MS.

Application Analytes:

Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate

Separation of Nine Nucleotides by Mixed-Mode Chromatography
  Nucleotides are important biological molecules which serve as subunits of nucleic acids. They are composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Nucleotides cannot be retained by reverse-phase chromatography without an ion-pairing reagent due to their highly polar nature. Primesep SB is capable of retaining and separating nine nucleotides. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups.

Application Analytes:

Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate
Guanosine Diphosphate
Guanosine Monophosphate
Guanosine Triphosphate

Separation of Nucleotide Monophosphates


Nucleotides are the monomers of DNA and RNA, composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Primesep SB is a good column for separating these highly polar compounds. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups. Retention can me manipulated by adjusting acetonitrile, and the baseline separation can be achieved in under 6 minutes using a gradient. 

Application Analytes:

Adenosine Monophosphate
Cytidine Monophosphate
Guanosine Monophosphate
Inosine Monophosphate
Uridine Monophosphate

HPLC Separation of Cytidine-5'-monophosphate (CMP) and 2'-Deoxycytidine 5'-Monophosphate (dCMP) on Newcrom B Column View on HPLC Separation of CMP and dCMP on Newcrom B Column_1193


Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 80/20%
Buffer H3PO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV 270nm


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds 2'-Deoxycytidine 5'-Monophosphate Cytidine-5'-monophosphate

Application Analytes:

2′-Deoxycytidine 5′-Monophosphate
Cytidine Monophosphate