Cytidine Triphosphate

CAS Number 81012-87-5
Molecular Formula C9H16N3O14P3
Molecular Weight 483.155 g/mol
LogP -5.6
  • Cytidine triphosphate
  • cytidine-5'-triphosphate
  • 65-47-4
  • Cytidine 5'-triphosphate
  • Cytidine 5'-(tetrahydrogen triphosphate)
  • CTP
  • Cytidine 3'-triphosphate
  • Deoxycytosine triphosphate
  • 5'-CTP
  • Cytidine 5'-triphosphoric acid
  • H4ctp
  • BRN 0071190
  • EINECS 200-611-4
  • CHEBI:17677
  • 81012-87-5
  • C9H16N3O14P3
  • [[(2R,3S,4R,5R)-5-(4-amino-2-oxo-pyrimidin-1-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate
  • 123334-07-6
  • cytidine 5'-triphosphate, disodium salt hydrate


HPLC Separation of Cytidines

Cytidine phosphates are nucleotides. Nucleotides contain a nucleobase, five-carbon sugar and one or three phosphate groups. All three cytidines are very polar and cannot be retained by reversed-phase chromatography without a ion-pairing reagent. Mixed-mode Primesep SB column easily allows to retain and separate mono-, di-, and tri-phospates by anion-exchange mechanism. Other nucleotides can be separated as well by this approach. Nucleotides in biofluids can be analyzed directly on this column without any significant sample preparation. Most of the proteins from biological fluids will be repelled from the surface of silica gel. Method is compatible with UV, ELSD, CAD and LC/MS.

Application Analytes:

Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate

Separation of Nine Nucleotides by Mixed-Mode Chromatography
  Nucleotides are important biological molecules which serve as subunits of nucleic acids. They are composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Nucleotides cannot be retained by reverse-phase chromatography without an ion-pairing reagent due to their highly polar nature. Primesep SB is capable of retaining and separating nine nucleotides. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups.

Application Analytes:

Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate
Guanosine Diphosphate
Guanosine Monophosphate
Guanosine Triphosphate