Deoxycytidine

CAS Number 652157-52-3
Molecular Formula C9H15N3O5
Molecular Weight 245.235 g/mol
InChI Key HXBGOHZLZCFWLH-OERIEOFYSA-N
LogP -1.84
Synonyms
  • 2'-Deoxycytidine hydrate
  • 2'-Deoxycytidine--water (1/1)
  • Cytidine, 2'-deoxy-, hydrate (1:1)
  • 652157-52-3

Applications:


Separation of Deoxyribo Nucleic Acid


Primesep 200 separates with baseline resolution the nucleosides (deoxyuridine, deoxyguanosine, deoxycytidine, deoxyadenozine) by a combination of cation exchange and reversed phase. These compounds are not retained on a traditional C18 column. Primesep 200 has an embedded anionic functional group which helps retain polar compounds by polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.

Application Analytes:


Deoxyadenosine
Deoxycytidine
Deoxyuridine

HPLC Separation of Nucleobases (2)

Nucleosides consisting of deoxyribose and a nucleobase cannot be separated on a common C18 column. By using a Primesep 200 mixed-mode column with a cation-exchange mechanism, nucleosides: deoxyuridine, deoxyguanosine, deoxycytidine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and TFA as buffer. UV detection at 270nm. 

Application Analytes:

Deoxyadenosine
Deoxycytidine
Deoxyuridine

HPLC Separation of Nucleosides and Deoxynucleosides

Th



Application Analytes:


Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Guanosine
Thymidine
Uridine