Primesep 100 separates a mixture of dicarboxylic acids in ion-exclusion mode with a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid (H2SO4) with UV detection at 210 nm. Baseline resolution of fumaric, maleic, malic, and succinic acids is obtained in less than 8 minutes. The separation combines ion-exclusion and reversed-phase mechanisms in one method.
Primesep 200 retains and separates the organic diacids (malic, succinic, fumaric, and maleic) by a combination hydrophobic, reversed-phase interactions and ion exclusion. The separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.
|Column||Primesep B, 4.6x150 mm, 5 µm, 100A|
|Flow Rate||1.0 ml/min|
|Detection||UV, 250 nm|
|Class of Compounds||Acid, Hydrophilic, Ionizable|
|Analyzing Compounds||Fumaric acid, Benzoic acid, Phthalic acid, Naphthoic acid, Maleic acid, )|