Epinephrine Sulfonate

Applications:


HPLC Separation of Epinephrine and Epinephrine Sulfonate
Epinephrine and epinephrine sulfonate are separated on combination of Primesep 100 and Primesep SB HPLC columns. Primesep 100 column retains epinephrine by combination of reversed-phase and cation-exchange mechanisms. Epinephrine sulfonate is retained by weak reversed-phase mechanism. Due to strong zwitter-ionic nature of epinephrine sulfonate, ionic interaction is not available for this molecule. Compounds can be monitored by UV, ELSD, CAD or LC/MS.

Condition

Column Primesep SB, Primesep 100 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 70/30%
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Epinephrine, Epinephrine Sulfonate
 

Application Analytes:

Epinephrine
Epinephrine Sulfonate

HILIC Separation of Epinephrine and Epinephrine Sulfonate
Epinephrine (also referred to as adrenaline) is a hormone and neurotransmitter. It is a catecholamine, a sympathomimetic monoamine derived from the amino acids phenylalanine and tyrosine. This method can be used to determine and quantitate epinephrine and epinephrine sulfonate in biological fluids (urine, blood, serum) and drug formulations. Obelisc N columns are used to retain and separate epinephrine and epinephrine sulfonate in mixed-mode hydrophilic interaction chromatography. Epinephrine is retained by the combination of cation-exchange and HILIC mechanisms. Epinephrine sulfonate is retained by HILIC mechanism. Buffer concentration and pH, as well as the amount of acetonitrile, can be used to adjust retention of both compounds. Both compounds can be detected by UV, ELSD and LC/MS. Preparative separation is possible with volatile mobile phases (ammonium formate or ammonium acetate.

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Epinephrine, Epinephrine Sulfonate
 

Application Analytes:

Epinephrine
Epinephrine Sulfonate

HPLC Analysis of Epinephrine and Epinephrine Sulfonate on Primesep AB Column
  Epinephrine and epinephrine sulfonate were analyzed using Primesep AB, a reverse-phase column with both acidic and basic embedded ion-pairing groups. This method is LC/MS compatible.  

Condition

Column Primesep AB, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer H3PO4 - 0.25%
Flow Rate 1.0 ml/min
Detection UV, 280 nm
 

Description

Class of Compounds Drug, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Epinephrine, Epinephrine Sulfonate
 

Application Analytes:

Epinephrine
Epinephrine Sulfonate