Fructose 6-Phosphate

CAS Number6814-87-5
Molecular FormulaC6H13O9P
Molecular Weight260.135
InChI KeyGSXOAOHZAIYLCY-HSUXUTPPSA-N
LogP-4.3
Synonyms
  • fructose-6-phosphate
  • Fructose 6-Phosphate
  • D-fructose-6-phosphate
  • fructose-6P
  • fructose-6-P
  • D-fructose-6-P
  • fru-6-P
  • 6814-87-5
  • FRUCTOSE -6-PHOSPHATE
  • keto-D-fructose 6-phosphate
  • F6R
  • FPC
  • D-fructose-6P
  • D-Fructose 6-phosphorate
  • D0H3IJ
  • Fructose 6-phosphoric acid
  • SCHEMBL8230
  • FRUCTOSE-6-PHOSPHONATE
  • AC1L2C15
  • AC1Q28E6
  • UNII-2012QM764Y
  • CHEBI:15946
  • CTK5C7413
  • GSXOAOHZAIYLCY-HSUXUTPPSA-N
  • Fructose 6-(dihydrogen phosphate)
  • Fructose, 6-(dihydrogen phosphate)

Applications:

HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column

November 30, 2022

HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Glucose 6-phosphate and  Fructose 6-phosphate on Newcrom B Column
HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column by SIELC Technologies.

Glucose 6-phosphate, also known as Robison ester, and Fructose 6-phosphate, known as Neuberg ester, are derivatives of glucose and fructose (respectively) that form during cellular metabolism. Glucose 6-phosphate is first formed by cells to ensure it does not diffuse out of the cell, and then through the process of glycolysis, it’s isomerized into Fructose 6-phosphate.

These 2 phosphorylated sugars can be retained, separated, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Formic acid (FA). This analytical method can be analyzed via any evaporative detector, including ELSD, CAD, and ESI-MS with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Glucose 6-phosphate and Fructose 6-phosphate


Condition

ColumnNewcrom B, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 50%
BufferFormic Acid – 0.5%
Flow Rate1.0 ml/min
DetectionELSD, 50C
Peak Retention Time11.4, 12.2 min

Description

Class of CompoundsSugar
Analyzing CompoundsGlucose 6-phosphate and Fructose 6-phosphate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Fructose 6-Phosphate
Glucose 6-Phosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Fructose 1,6 Bisphosphate on Newcrom B Column

May 13, 2021

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate

Fructose 1,6-biphosphate, alternatively known as Harden-Young ester, is the main form glucose and fructose take when they enter cells. It is a key component of the glycolysis metabolic pathway and is the product of the phosphorylation of fructose 6-phosphate.
Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile with an ammonium formate (AmFm) buffer, Fructose 1,6-bisphosphate can be retained, separated (from fructose 6-phosphate), and measured. Fructose 1,6 bisphosphate can be detected using charged aerosol detection (CAD) with high resolution.

Condition

Column Newcrom B, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm – pH 3.0 – 20 mm
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
Sugar Phosphate
Analyzing Compounds Sodium, Fructose 6 – phosphate, Fructose 1.6 biphosphate

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Fructose 1,6 Bisphosphate
Fructose 6-Phosphate
Phosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Sugar Phosphates

October 14, 2010


Sugar phosphates occur in biological systems. Sugar phosphates are parts of two metabolic pathways: glycolisis and pentose phosphate pathways. One of the purposes of sugar phosphates is to store and transfer energy in cells. Because of the presence of sugar fragment and phosphate moiety these molecules are very polar in nature. Mixed-mode chromatography was applied for separation of glucose and fructose phosphates and diphosphates. Compounds are separated based on anion-exchange properties with diphosphates retaining longer than mono phosphates. Monitoring is done by ELSD.

 

 

Condition

Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm – pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Sugars
Analyzing Compounds Sodium, Glucose 6- phosphate, Fructose 6 –phosphate, Fructose 1.6 biphosphate, Ribulose 1.5 biphosphate

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Fructose 1,6 Bisphosphate
Fructose 6-Phosphate
Glucose 6-Phosphate
Ribulose 1,5 Bisphosphate
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.