Glucose 6-Phosphate

CAS Number 299-31-0
Molecular Formula C6H13O9P
Molecular Weight 260.135 g/mol
InChI Key NBSCHQHZLSJFNQ-GASJEMHNSA-N
LogP -4.2
Synonyms
  • Robison ester
  • Glucose 6-phosphate
  • 6-O-phosphono-D-glucopyranose
  • D-glucopyranose 6-phosphate
  • D-glucopyranose, 6-(dihydrogen phosphate)
  • Glucose 6-(dihydrogen phosphate)
  • 299-31-0
  • D-Glucose, 6-(dihydrogen phosphate)
  • {[(2R,3S,4S,5R)-3,4,5,6-tetrahydroxyoxan-2-yl]methoxy}phosphonic acid
  • D-glucose-6P
  • Glc6P
  • D-glucose-6-P
  • a-D-glucose 6- phosphate
  • D05TIS
  • AC1L1LI0
  • SCHEMBL48379
  • alpha-D-Glucose 6- phosphate

Applications:


HPLC Separation of Sugar Phosphates
Sugar phosphates occur in biological systems. Sugar phosphates are parts of two metabolic pathways: glycolisis and pentose phosphate pathways. One of the purposes of sugar phosphates is to store and transfer energy in cells. Because of the presence of sugar fragment and phosphate moiety these molecules are very polar in nature. Mixed-mode chromatography was applied for separation of glucose and fructose phosphates and diphosphates. Compounds are separated based on anion-exchange properties with diphosphates retaining longer than mono phosphates. Monitoring is done by ELSD.    

Condition

Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm - pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Sugars
Analyzing Compounds Sodium, Glucose 6- phosphate, Fructose 6 –phosphate, Fructose 1.6 biphosphate, Ribulose 1.5 biphosphate
 

Application Analytes:

Fructose 1,6 Bisphosphate
Fructose 6-Phosphate
Glucose 6-Phosphate
Ribulose 1,5 Bisphosphate
Sodium

HPLC Separation of Glucose 6- Phosphate and Glucose 1- Phosphate on Newcrom B Column
HPLC.cloud View on hplc.cloud Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a reverse-phase mixed-mode anion-exchange Newcrom B column. What differentiates, the Newcrom B phase from other silica-based Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic Acid -  3%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Hydrophilic,  Sugar
Analyzing Compounds Glucose 6- Phosphate,  Glucose 1- Phosphate


Application Analytes:

Glucose 1- Phosphate
Glucose 6-Phosphate

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