| CAS Number | 6899-04-3 |
|---|---|
| Molecular Formula | C5H10N2O3 |
| Molecular Weight | 146.146 g/mol |
| InChI Key | ZDXPYRJPNDTMRX-UHFFFAOYSA-N |
| LogP | -2.95 |
| Synonyms |
|
Applications:
HPLC Separation of Amino Acids in Supplements Capsules
Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.
| Column | Primesep 100, 4.6x250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 30/70% |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Arginine, Glutamine, L-Valine (Val/V), L-Isoleucine (Ile/I), L-Leucine (Leu/L) |
Application Analytes:
Arginine
Glutamine
Isoleucine
Leucine
Valine
