Glutamine

Glutamine

CAS Number 6899-04-3
Molecular Formula C5H10N2O3
Molecular Weight 146.146 g/mol
InChI Key ZDXPYRJPNDTMRX-UHFFFAOYSA-N
LogP -2.95
Synonyms
  • DL-Glutamine
  • Glutamine
  • 6899-04-3
  • Glutamine
  • EINECS 230-006-0
  • (+-)-Glutamine
  • gamma-Glutamine
  • Glutamine DL-form
  • NSC 97925
  • 2,5-diamino-5-oxopentanoic acid
  • 2-amino-4-carbamoylbutanoic acid
  • Glutamin
  • Glutaminsaeure-5-amid
  • Hgln
  • glutamic acid gamma-amide
  • 585-21-7

Applications:

HPLC Separation of Amino Acids in Supplements Capsules
Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.

Condition
Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, Glutamine,  L-Valine (Val/V),  L-Isoleucine (Ile/I), L-Leucine (Leu/L)
 
Application Analytes:

Arginine
Glutamine
Isoleucine
Leucine
Valine