Glycine

Glycine

CAS Number 56-40-6
Molecular Formula C2H5NO2
Molecular Weight 75.067 g/mol
InChI Key DHMQDGOQFOQNFH-UHFFFAOYSA-N
LogP -3.21
Synonyms
  • Glycine
  • 56-40-6
  • Amino-acetic acid
  • 2-Aminoacetic acid
  • Acetic acid, amino-
  • Aciport
  • Aminoacetic acid
  • AMINOETHANOIC ACID
  • glicina-sulfato de hierro (1:1)
  • Glicoamin
  • Glycocoll
  • Glycolixir
  • Glycosthene
  • Gyn-Hydralin
  • NSC 25936
  • NSC 2916
  • NSC 54188
  • Amitone
  • EINECS 200-272-2
  • FEMA No. 3287
  • GLY
  • L-Glycine
  • Glycine, non-medical
  • Hampshire glycine
  • Sucre de gelatine
  • Aminoazijnzuur
  • Aminoacetic acid 1.5% in plastic container
  • Glycine 1.5% in plastic container
  • Acide aminoacetique
  • Acido aminoacetico
  • Acidum aminoaceticum
  • Glicina
  • Glycinum
  • UNII-TE7660XO1C
  • 2-Aminoacetate
  • 2-aminoethanoic acid
  • Amino-Acetate
  • Aminoacetate
  • Aminoessigsaeure
  • Aminoethanoate
  • Glycin
  • Glycosthene
  • Glykokoll
  • Glyzin
  • H2N-CH2-COOH
  • Hgly
  • Leimzucker
  • Padil
  • glycine zwitterion
  • 1119449-36-3
  • 1173020-11-5
  • 1196157-78-4
  • 52955-63-2
  • 57678-19-0
  • 848646-45-7
  • 87867-94-5
  • 1153953-88-8

Applications:


HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography
Dimethyl sulfoxide is important polar aprotic solvent, which is frequently used in pharmaceutical drug manufacturing, desolution, etc. DMSO is used as one of the solvents on protein chemistry due to universal ability to dissolve small molecules like amino acids. Amino acids and DMSO are very polar and have no retention on reversed-phase columns. In this HPLC application DMSO and glycine are separated on Primesep 100 mixed-mode column. DMSO is retained by weak reversed-phase mechanism and very low organic concentration is required in order to achieve any retention. Glycine, like any other underivatized amino acid, is retained by weak reversed-phase and moderate cation-exchange mechanism. Method uses acetonitrile/water/TFA gradient and UV detection.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds DMSO, Glycine


Application Analytes:

Amino Acids
DMSO (Dimethyl sulfoxide)
Glycine

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar
Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Condition

Column Primesep D, 3.2x50 mm, 5 µm, 100A
Mobile Phase IPA
Buffer Formic Acid
Flow Rate 0.5 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Glycine, Histidine, Sucrose, Polysorbate 20


Application Analytes:

Amino Acids
Glycine
Histidine
Polysorbate
Polysorbate 20
Polysorbate 80
Sucrose
Tween 20
Tween 80

HPLC Separation of Lysine and Arginine from Other Amino Acids
  Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge. Application Columns: Primesep C Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 15%
Buffer AmAc pH 5.0- 15 mM
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
 

Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride
HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride _1146  

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer H2SO4 - 0.5%
Flow Rate 1 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds  Amino acid, Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Zwitterionic
Analyzing Compounds Glycine,  Glycine Methyl Ester
 

Application Analytes:

Glycine
Glycine methyl ester

HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride
 

Condition

Column Primesep A, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H2SO4 - 0.05%
Flow Rate 0.05 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds  Amino acid, Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Zwitterionic
Analyzing Compounds Glycine,  Glycine Methyl Ester
 

Application Analytes:

Glycine
Glycine methyl ester

HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Glutamin (Gln/Q), L-Glycine (Gly/G), L-Cysteine (Cys/C), L-Proline Pro/p), L-Tyrosine (Tyr/Y), L-Arginine (Arg/R)
 

Application Analytes:

Amino Acids
Arginine
Cysteine
Glycine
L-Cysteine
L-Glutamine
Proline
Tyrosine

New HPLC Amino Acids Separation Compatible With Carbon Dating Technique
   

Hydroxyproline seems to be the most promising amino acid used in carbon dating when isolated from bone collagen. Separation of amino acids is challenging, especially without the use of ions or inorganic buffers that can interfere with Mass spectrometer (MS) or contaminate the sample with modern carbon. Amino acids are also not retained in reverse-phase chromatography. The ideal solution would be using water only to separate the amino acids. This would allow a direct coupling to MS. We were able to separate hydroxyproline from proline and other simple amino acids like glycine and alanine in HPLC on Newcrom AH column using water only as a mobile phase. Using water also allowed UV detection at 205 nm which can’t be done if using a buffer based on acetic or formic acid. See more information on radiocarbon dating here. The same method can be modified to get symmetrical peaks and higher efficiency if a mobile phase with ionic modifier such as formic acid is used.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Flow Rate 1.0 ml/min
Detection UV, 205 nm, CAD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Alanine, Glycine, Proline, Hydroxyproline
 

Application Analytes:

Alanine
D-Alanine
Glycine
Hydroxyproline
Proline

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