Glycine

CAS Number 56-40-6
Molecular Formula C2H5NO2
Molecular Weight 75.067 g/mol
InChI Key DHMQDGOQFOQNFH-UHFFFAOYSA-N
LogP -3.21
Synonyms
  • Glycine
  • 56-40-6
  • Amino-acetic acid
  • 2-Aminoacetic acid
  • Acetic acid, amino-
  • Aciport
  • Aminoacetic acid
  • AMINOETHANOIC ACID
  • glicina-sulfato de hierro (1:1)
  • Glicoamin
  • Glycocoll
  • Glycolixir
  • Glycosthene
  • Gyn-Hydralin
  • NSC 25936
  • NSC 2916
  • NSC 54188
  • Amitone
  • EINECS 200-272-2
  • FEMA No. 3287
  • GLY
  • L-Glycine
  • Glycine, non-medical
  • Hampshire glycine
  • Sucre de gelatine
  • Aminoazijnzuur
  • Aminoacetic acid 1.5% in plastic container
  • Glycine 1.5% in plastic container
  • Acide aminoacetique
  • Acido aminoacetico
  • Acidum aminoaceticum
  • Glicina
  • Glycinum
  • UNII-TE7660XO1C
  • 2-Aminoacetate
  • 2-aminoethanoic acid
  • Amino-Acetate
  • Aminoacetate
  • Aminoessigsaeure
  • Aminoethanoate
  • Glycin
  • Glycosthene
  • Glykokoll
  • Glyzin
  • H2N-CH2-COOH
  • Hgly
  • Leimzucker
  • Padil
  • glycine zwitterion
  • 1119449-36-3
  • 1173020-11-5
  • 1196157-78-4
  • 52955-63-2
  • 57678-19-0
  • 848646-45-7
  • 87867-94-5
  • 1153953-88-8

Applications:


HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography



Dimethyl sulfoxide is important polar aprotic solvent, which is frequently used in pharmaceutical drug manufacturing, desolution, etc. DMSO is used as one of the solvents on protein chemistry due to universal ability to dissolve small molecules like amino acids. Amino acids and DMSO are very polar and have no retention on reversed-phase columns. In this HPLC application DMSO and glycine are separated on Primesep 100 mixed-mode column. DMSO is retained by weak reversed-phase mechanism and very low organic concentration is required in order to achieve any retention. Glycine, like any other underivatized amino acid, is retained by weak reversed-phase and moderate cation-exchange mechanism. Method uses acetonitrile/water/TFA gradient and UV detection.



Application Analytes:

Amino Acids
DMSO (Dimethyl sulfoxide)
Glycine

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar


Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Application Analytes:

Amino Acids
Glycine
Histidine
Polysorbate
Polysorbate 20
Polysorbate 80
Sucrose
Tween 20
Tween 80

HPLC Separation of Lysine and Arginine from Other Amino Acids

 

Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge.

Application Columns: Primesep C
Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
Detection technique: UV, LC/MS, ELSD/CAD



Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride
HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride _1146

Application Analytes:

Glycine
Glycine methyl ester

HPLC Method for Analysis of Glycine Methyl Ester Hydrochloride


Application Analytes:

Glycine
Glycine methyl ester

HPLC Separation of Mixture of Conditionally Essential Amino Acids


Application Analytes:

Amino Acids
Arginine
Cysteine
Glycine
L-Cysteine
L-Glutamine
Proline
Tyrosine