Guanosine

CAS Number 118-00-3
Molecular Formula C10H13N5O5
Molecular Weight 283.244 g/mol
InChI Key NYHBQMYGNKIUIF-UUOKFMHZSA-N
LogP -2.7
Synonyms
  • guanosine
  • 118-00-3
  • guanine riboside
  • vernine
  • Guanozin
  • Guanosin
  • Inosine, 2-amino-
  • USAF CB-11
  • Vernine (VAN)
  • L-GUANOSINE
  • 9-beta-D-Ribofuranosylguanine
  • Guanine-9-beta-D-ribofuranoside
  • beta-D-Ribofuranoside, guanine-9
  • Guanine, 9-beta-D-ribofuranosyl-
  • 2(3H)-Imino-9-beta-D-ribofuranosyl-9H-purin-6(1H)-one
  • Inosine, 2-amino- (VAN)
  • Ribofuranoside, guanine-9, beta-D-
  • 2-Amino-1,9-dihydro-9-beta-D-ribofuranosyl-6H-purin-6-one
  • GUANINE-9:BETA-D-RIBOFURANOSIDE
  • Guo
  • AI3-52065
  • NSC 19994
  • 9-beta-D-ribofuranosyl-guanine
  • Guanine, 9-beta-D-ribofuranosyl- (VAN)

Applications:


HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N

Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm.

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN -90%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Uracil, Uridine, Adenosine, Guanosine, Cytidine, Cytosine
   

Application Analytes:

Adenosine
Cytidine
Cytosine
Guanosine
Uracil
Uridine

HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method
  Application Notes: Nucleosides are glycosylamines consisting of nucleobase linked to ribose or deoxyribose sugar and are building blocks for DNA and RNA. These compounds are very polar and contain groups available for hydrogen bonding interaction. Thymidine, uridine, adenosine, guanosine and cytidine were separated using a hydrogen-bonding method. There is a strong correlation between the retention time and mobile phase composition. The strength of hydrogen-bonding interaction increases as the number of hydroxyls in the analytes increase. Additionally the rder of elution for compounds depends on the ratio of the mobile phases: acetonitrile and methanol. Our method is compatible with LC/MS and preparative chromatography. Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site. Application Compounds: Thymidine, uridine, adenosine, guanosine and cytidine

Condition

Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Adenosine, Guanosine, Cytidine
   

Application Analytes:

Adenosine
Cytidine
Guanosine
Thymidine
Uridine

HPLC Separation of Nucleosides and Deoxynucleosides

Condition

Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine
 

Application Analytes:

Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Guanosine
Thymidine
Uridine

HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column
Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine
 

Application Analytes:

Adenosine
Deoxyadenosine
Deoxyguanosine
Guanosine