Guanosine

Applications:

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HPLC Separation of Nucleic Bases at pH 4 and 5 on Obelisc N

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Nucleic bases are biological compounds found in genetic molecules (DNA, RNA). They can be separated on an Obelisc N column, which offers very polar characteristics and can be used with positively or negatively charged groups. Closely-eluted adenosine and uridine can be further separated by simply adjusting the pH of the mobile phase. Mobile phase is water and acetonitrile (MeCN, ACN) with Ammonium Acetate as buffer. UV detection at 250nm.

Condition

Column	Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN -90%
Buffer	AmAc
Flow Rate	1.0 ml/min
Detection	UV, 250 nm

 

Description

Class of Compounds	Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds	Uracil, Uridine, Adenosine, Guanosine, Cytidine, Cytosine

   

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Adenosine
Cytidine
Cytosine
Guanosine
Uracil
Uridine

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HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method

  Application Notes: Nucleosides are glycosylamines consisting of nucleobase linked to ribose or deoxyribose sugar and are building blocks for DNA and RNA. These compounds are very polar and contain groups available for hydrogen bonding interaction. Thymidine, uridine, adenosine, guanosine and cytidine were separated using a hydrogen-bonding method. There is a strong correlation between the retention time and mobile phase composition. The strength of hydrogen-bonding interaction increases as the number of hydroxyls in the analytes increase. Additionally the rder of elution for compounds depends on the ratio of the mobile phases: acetonitrile and methanol. Our method is compatible with LC/MS and preparative chromatography. Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site. Application Compounds: Thymidine, uridine, adenosine, guanosine and cytidine

Condition

Column	Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase	MeCN/MeOH
Buffer	AmFm, Formic acid
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

 

Description

Class of Compounds	Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds	Thymidine, Uridine, Adenosine, Guanosine, Cytidine

   

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Adenosine
Cytidine
Guanosine
Thymidine
Uridine

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HPLC Separation of Nucleosides and Deoxynucleosides

Condition

Column	Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN/MeOH
Buffer	AmFm, Formic acid
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

 

Description

Class of Compounds	Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds	Thymidine, Uridine, Deoxyadenosine, Adenosine, Deoxyguanosine, Guanosine, Deoxycytidine, Cytidine

 

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Adenosine
Cytidine
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Guanosine
Thymidine
Uridine

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HPLC Separation of Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine on Newcrom AH Column

Separation type: Liquid Chromatography Mixed-mode View on hplc.cloud Nucleosides are the building blocks for DNA and RNA as well as other roles in biomechanical processes such as signal transduction. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, nucleosides: guanosine, deoxyguanosine, adenosine, and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and H3PO4 as a buffer. UV detection at 210 nm.

Condition

Column	Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O - 20/80%
Buffer	H3PO4 - 0.5%
Flow Rate	1.0 ml/min
Detection	UV, 210 nm

 

Description

Class of Compounds	Nucleoside,  Hydrophilic, Ionizable
Analyzing Compounds	Guanosine, Deoxyguanosine, Adenosine, Deoxyadenosine

 

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Adenosine
Deoxyadenosine
Deoxyguanosine
Guanosine

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