Hyoscyamine Sulfate

CAS Number 620-61-1
Molecular Formula C17H25NO7S
Molecular Weight 387.447 g/mol
  • Anaspaz
  • Atropine Sulfate, 3(S)-endo-Isomer
  • Atropine, 3(S)-endo-Isomer
  • Cytospaz
  • Hyoscyamine
  • Hyoscyamine Hydrobromide
  • Hyoscyamine Hydrochloride
  • Hyoscyamine Sulfate
  • Hyoscyamine Sulfate Anhydrous


HPLC Separation of Active Compounds in Drug Formulation
HPLC Separation of Active Compounds in Drug Formulation
An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.

Application Analytes:

Benzoic Acid
Hyoscyamine Sulfate

HPLC Method for Analysis of Hyoscyamine
77 Hyoscyamine is a natural plant belladonna alkaloid derivative and the levorotatory form of racemic atropine. It functions as a non-selective, competitive antagonist of muscarinic receptors and is used to treat a variety of stomach pains and intestinal problems as well as to decrease side effects of certain medications and insecticides. The drug does that by decreasing the acid production in the stomach and slowing down the natural movements of the gut. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Hyoscyamine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.  


Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN - 40%
Buffer H3PO4 - 0.1%,
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds Drug,  Acid, Hydrophilic, Ionizable, Supplements
Analyzing Compounds Hyoscyamine

Application Analytes:

Hyoscyamine Sulfate