Iso-Alpha Acids

Iso-Alpha Acids

Synonyms

    Applications:

    HPLC Analysis of Hop Extract (Beer Brewing)

    October 14, 2010


    Hop extract is a complex mixture of alpha and beta acids, essential oils, tannins, and waxes. Components of the hop extract were separated by mixed-mode HPLC on a Primesep SB column. Compounds on this column are retained by combination of reversed-phase and anion-exchange mechanism. At higher pH (3-5), alpha and beta acids are ionized. Retention time can be controlled by amount of acetonitrile, buffer pH, and buffer concentration. Primesep SB column is fully ionized within operational range of the column.

    Application Column

    Primesep SB

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Iso-Alpha Acids
    Tetrahydro-Iso-Alpha Acids

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.