Leucine

Leucine

CAS Number 61-90-5
Molecular Formula C6H13NO2
Molecular Weight 131.175 g/mol
InChI Key ROHFNLRQFUQHCH-YFKPBYRVSA-N
LogP -1.52
Synonyms
  • Leucine
  • L-Leucine
  • 61-90-5
  • L-Leucine
  • (2S)-2-Amino-4-methylpentanoic acid
  • (S)-(+)-Leucine
  • (S)-2-Amino-4-methylpentanoic acid
  • (S)-2-Amino-4-methylvaleric acid
  • (S)-Leucine
  • L-(+)-Leucine
  • LEUCINE, L-
  • L-Leucin
  • L-leucina
  • L-Norvaline, 4-methyl-
  • L-α-Aminoisocaproic acid
  • NSC 46709
  • Pentanoic acid, 2-amino-4-methyl-, (S)-
  • L-(-)-2-Amino-4-methylpentanoic acid
  • 2-Amino-4-methylpentanoic acid, (S)-
  • EINECS 200-522-0
  • FEMA No. 3297
  • Valeric acid, 2-amino-4-methyl-, (S)-
  • Leucina
  • Leucinum
  • UNII-GMW67QNF9C
  • (2S)-2-Amino-4-methylpentanoate
  • (2S)-2-amino-4-methyl-pentanoic acid
  • (S)-2-Amino-4-methylpentanoate
  • (S)-2-Amino-4-methylvalerate
  • 4-Methyl-L-Norvaline
  • L-Leuzin
  • L-a-Aminoisocaproate
  • L-a-Aminoisocaproic acid
  • L-alpha-Aminoisocaproate
  • L-alpha-Aminoisocaproic acid
  • leucine zwitterion
  • L-leucine zwitterion
  • 7005-03-0

Applications:


Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 195 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Aspartic acid,  Alanine, Valine, Methionine, Leucine
 

Application Analytes:

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

HPLC Separation of Amino Acids in Supplements Capsules
Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, Glutamine,  L-Valine (Val/V),  L-Isoleucine (Ile/I), L-Leucine (Leu/L)
 

Application Analytes:

Arginine
Glutamine
Isoleucine
Leucine
Valine

HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column
Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis.

Condition

Column Primesep 200, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Na2HPO4
Flow Rate 1.0 ml/min
Detection UV 210 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Valine, Leucyne, Isoleycine, Tyrosine, Phenylalanine, Histidine, Lysine
 

Application Analytes:

Histidine
Isoleucine
Leucine
Lysine
Phenylalanine
Tyrosine
Valine

HPLC Separation of Lysine and Arginine from Other Amino Acids
  Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge. Application Columns: Primesep C Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 15%
Buffer AmAc pH 5.0- 15 mM
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
 

Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R)
 

Application Analytes:

Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
Leucine
Methionine
Phenylalanine
Proline
Tryptophan
Valine

HPLC Separation of β‑Hydroxy-β-methylbutyric Acid, 2-Hydroxyisocaproic Acid and Leucine on Primesep 100 Column
Both β-hydroxy-β-methylbutyric acid (HMB) and 2-hydroxyisocaproic acid (HICA) are metabolites of the essential dietary branched chain amino acid leucine.
 

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 5%, 3 min hold, Gradient MeCN 5-30% 12 min
Buffer H2SO4 - 0.05 % 3 min hold, GradientH2SO4 0.05-3% 12 min
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Acid, Hydrophilic,  Ionizable, Normal human metabolite, Dietary supplement, Essential amino acid, Branched-chain amino acid (BCAA)
Analyzing Compounds β‑Hydroxy-β-methylbutyric (HMB), 2-Hydroxyisocaproic Acid (HICA), Leucine
 

Application Analytes:

2-Hydroxyisocaproic Acid (HICA)
D-Leucine
Leucine
β‑Hydroxy-β-methylbutyric Acid (HMB)

HPLC Separation of Allo-Isoleucine, Isoleucine, Leucine on Primesep 100 Column
HPLC.cloud View on hplc.cloud HPLC Separation of Allo-Isoleucine  Isoleucine, Leucine on Primesep 100 Column Chr_1197

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFM pH 3.0 - 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Allo-Isoleucine, Isoleucine, Leucine
 

Application Analytes:

D-Isoleucine
D-Leucine
DL-Isoleucine
Isoleucine
L-Alloisoleucine
L-Isoleucine
Leucine
allo-D-isoleucine

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