Leucine

CAS Number 61-90-5
Molecular Formula C6H13NO2
Molecular Weight 131.175 g/mol
InChI Key ROHFNLRQFUQHCH-YFKPBYRVSA-N
LogP -1.52
Synonyms
  • Leucine
  • L-Leucine
  • 61-90-5
  • L-Leucine
  • (2S)-2-Amino-4-methylpentanoic acid
  • (S)-(+)-Leucine
  • (S)-2-Amino-4-methylpentanoic acid
  • (S)-2-Amino-4-methylvaleric acid
  • (S)-Leucine
  • L-(+)-Leucine
  • LEUCINE, L-
  • L-Leucin
  • L-leucina
  • L-Norvaline, 4-methyl-
  • L-α-Aminoisocaproic acid
  • NSC 46709
  • Pentanoic acid, 2-amino-4-methyl-, (S)-
  • L-(-)-2-Amino-4-methylpentanoic acid
  • 2-Amino-4-methylpentanoic acid, (S)-
  • EINECS 200-522-0
  • FEMA No. 3297
  • Valeric acid, 2-amino-4-methyl-, (S)-
  • Leucina
  • Leucinum
  • UNII-GMW67QNF9C
  • (2S)-2-Amino-4-methylpentanoate
  • (2S)-2-amino-4-methyl-pentanoic acid
  • (S)-2-Amino-4-methylpentanoate
  • (S)-2-Amino-4-methylvalerate
  • 4-Methyl-L-Norvaline
  • L-Leuzin
  • L-a-Aminoisocaproate
  • L-a-Aminoisocaproic acid
  • L-alpha-Aminoisocaproate
  • L-alpha-Aminoisocaproic acid
  • leucine zwitterion
  • L-leucine zwitterion
  • 7005-03-0

Applications:


Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Application Analytes:

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

HPLC Separation of Amino Acids in Supplements Capsules



Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.



Application Analytes:

Arginine
Glutamine
Isoleucine
Leucine
Valine

HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column

Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis.



Application Analytes:

Histidine
Isoleucine
Leucine
Lysine
Phenylalanine
Tyrosine
Valine

HPLC Separation of Lysine and Arginine from Other Amino Acids

 

Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge.

Application Columns: Primesep C
Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
Detection technique: UV, LC/MS, ELSD/CAD



Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine