Applications:
Reverse Elution Order in Separation of Alkali Cation

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
Condition
Column |
Primesep C, 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
AmAc |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Lithium, Chloride |
LithiumPotassiumSodium
Complex of Cations on Primesep C is Effected by Organic Modifier

Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
Condition
Column |
Primesep C, 4.6x50 mm, 5 µm, 100A |
Mobile Phase |
MeOH, MeCN |
Buffer |
AmAc pH 5.0 |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Lithium, Chloride |
LithiumPotassiumSodium
Separation of Metal Ions on Primesep 100 Column

Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
Condition
Column |
Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
TFA |
Flow Rate |
1.0 ml/min |
Detection |
ELSD 50C |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Phosphate, Magnesium, Calcium, Lithium, Zinc |
CalciumChlorideLithiumMagnesiumPotassiumSodiumZinc
Separation of Sodium, Potassium, Lithium, and Ammonium in Mixed-Mode HPLC
Inorganic cations and ammonia are usually analyzed by ion-exchange chromatography with a conductivity detector. Mixed-mode chromatography achieves the same goal with an ELSD detector. In addition to retaining ions, mixed-mode column can retain hydrophobic molecules also, making it possible to analyze a wide variety of ionic and non-ionic compounds in one run. In this method sodium, potassium, and lithium ions were separated along with ammonium ions on a Primesep 100 column. Detection technique is ELSD.
Condition
Column |
Primesep 100, 4.6x250 mm, 5 µm, 100A |
Mobile Phase |
MeCN/H2O |
Buffer |
TFA |
Flow Rate |
1.0 ml/min |
Detection |
ELSD |
Description
Class of Compounds
|
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
Analyzing Compounds |
Sodium, Potassium, Bromide, Ammonium |
Ammonium IonLithiumPotassiumSodium