Molecular Formula C4H2O4
Molecular Weight 114.056 g/mol
  • maleate
  • (Z)-2-Butenedioate
  • (Z)-but-2-enedioate
  • (2Z)-but-2-enedioate
  • male


HPLC Separation of Drugs in Tylenol Cold and Cough Remedies
Components of Tylenol Cold and Cough Remedy are separated on Obelisc R mixed-mode column. Method can be used to determine compounds in various cough and cold compositions. Neutral (acetaminophen), basic (chlorpheniramine, dextromethorphan and pseudoephedrine) and acidic (maleic acid/Maleate) components are analyzed with perfect peak shape and retention control. Method can be used in production, QC/QA and biological studies for quantitation of various components in pharmaceutical formulations (Advil, Tylenol, Dimetapp, Robitussin, NyQuil, etc)


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable, Neutral, Basic
Analyzing Compounds Maleate, Chlorpheniramine, Dextromethorphan, Pseudoephedrine,  Acetaminophen

Application Analytes:

Acetaminophen (Paracetamol)
Pseudoephedrine (PSE)

HPLC Separation of Drugs in Advil Allergy and Sinus

Ibuprofen is in a group of drugs called non-steroidal anti-inflammatory drugs (NSAIDs). Ibuprofen works by reducing hormones that cause inflammation and pain in the body.
Pseudoephedrine is a decongestant that shrinks blood vessels in the nasal passages. Dilated blood vessels can cause nasal congestion (stuffy nose).
The combination of chlorpheniramine, ibuprofen, and pseudoephedrine is used to treat sneezing, itching, watery eyes, runny nose, stuffy nose, sinus congestion, cough, and pain or fever caused by the common cold or flu in common over-the-counter medication (Advil, NyQuil, Tylenol, etc.)
Active ingredients of Advil Allergy and Sinus composition are separated by combination of reverse phase, cation-exchange and anion exchange mechanisms. Obelisc R mixed-mode column is used to separate pseudoephedrine (polar basic compound), chlorpheniramine (hydrophobic basic compound), ibuprofen (hydrophobic acidic compound) and maleic acid (counter-ion, hydrophilic acid compound). Method can be used for QC in production, impurity profiling and research and development. All compounds are well separated with high efficiency and good peak shape. Method uses ammonium formate buffer but other buffers can be used as well (ammonium acetate, sodium and ammonium phosphate) with corresponding detection technique - ELSD, LC/MS, and UV. Chlorpheniramine is an antihistamine that reduces the natural chemical histamine in the body. Histamine can produce symptoms of sneezing, itching, watery eyes, and runny nose.

Application Analytes:

Pseudoephedrine (PSE)

HPLC Separation of Acetaminophen, Caffeine and Pyrilamine maleate
Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever. Caffeine is a Central Nervous System (CNS) stimulant. It is an unregulated and legal drug in most parts of the world. It can be found in the seeds and leaves in a number of plants native in Africa, East Asia and South America. Pyrilamine maleate is a histamine H1 antagonist with hypnotic properties. It has multiple uses such as an anesthetic and is used against allergies. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain Acetaminophen, Caffeine and Pyrilamine maleate. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Analytes:

Acetaminophen (Paracetamol)
Pyrilamine maleate