Methanesulfonic Acid

CAS Number 75-75-2
Molecular Formula CH4O3S
Molecular Weight 96.1 g/mol
LogP -0.9
  • 75-75-2
  • Methylsulfonic acid
  • Methanesulphonic acid
  • Methanesulfonicacid
  • Kyselina methansulfonova
  • Methansulfonsaeure
  • NSC 3718
  • CCRIS 2783
  • UNII-12EH9M7279
  • Kyselina methansulfonova [Czech]
  • HSDB 5004
  • EINECS 200-898-6
  • CH3SO3H
  • BRN 1446024


Separation of Sulphonium Ions in Polar Organic Mode

The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).

Application Analytes:

Methanesulfonic Acid
Sulfonium Ions

HPLC Separation of Inorganic Anions

Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.

Application Analytes:

Hydrochloric Acid
Methanesulfonic Acid
Nitric Acid
Organic Acids
Perchloric Acid
TFA (Trifluoroacetic Acid)

HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N

Ion chromatography is usually used for analysis of hydrophilic organic and inorganic ions. Same separation can be achieved on HILIC/mixed-mode Obelisc N HPLC columns. Obelisc N HPLC columns have very polar groups on their surface: one of the groups is basic and the other acidic. In case of low organic concentration, two groups are connected by hydrophilic linker. Obelisc N column can be used as cation-exchange and anion-exchange column. This allows to separate positively and negatively charged molecules in one run. Five anions (chloride, bromide, methanesulfonate, nitrate and perchlorate) along with one cation (sodium) were separated in one run. Method is compatible with ELSD, CAD and LC/MS and can be used for analysis of various hydrophilic and hydrophobic cations and anions in one HPLC run.

Application Analytes:

Methanesulfonic Acid
Nitric Acid

The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep B2 mixed-mode column. Primesep B2 is a reverse-phase column with embedded basic ion-pairing groups.
The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep SB mixed-mode column. Primesep SB is a reverse-phase column with embedded basic ion-pairing groups.  


Column Primesep SB, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer Gradient AmFm pH 3.0- 2-50 mM, 3 min, 3 min hold
Flow Rate 1.0 ml/min
Detection CAD


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glucuronic acid, Methanesulfonic acid, Butanesulfonic acid

Application Analytes:

Benzenesulfonic Acid
Butanesulfonic acid
Glucuronic acid
Methanesulfonic Acid