Methionine

CAS Number 59-51-8
Molecular Formula C5H11NO2S
Molecular Weight 149.210 g/mol
InChI Key FFEARJCKVFRZRR-UHFFFAOYSA-N
LogP -0.978
Synonyms
  • dl-Methionine
  • Methionine
  • 59-51-8
  • 2-amino-4-(methylsulfanyl)butanoic acid
  • (.+-.)-Methionine
  • Acimetion
  • Banthionine
  • Cynaron
  • DDL METHIONINE
  • DL-2-Amino-4-(methylthio)butyric acid
  • DL-2-AMINO-4-METHYLTHIOBUTANOIC ACID
  • DL-2-Amino-4-methylthiobutyric acid
  • DL-Methionin
  • DL-metionina
  • Lobamine
  • Meonine
  • Meprom M 85
  • Methilanin
  • METHIONINE, DL-
  • METHIONINE, DL-, DEGUSSA
  • Metione
  • NSC 9241
  • Pedameth
  • Racemethionine
  • Rhodimet NP 99
  • Urimeth
  • DL-2-Amino-4-(methylthio)butanoic acid
  • alpha-Amino-gamma-(methylthio)butyric acid
  • DL-2-Amino-4-(methylthio)-butyric acid
  • EINECS 200-432-1
  • FEMA No. 3301
  • Mertionin
  • (+-)-Methionine
  • Methionine, amorphous
  • DL-Methioninum
  • UNII-73JWT2K6T3
  • 2-amino-4-(methylthio)butanoic acid
  • Hmet
  • M
  • Met
  • Methionin
  • alpha-amino-gamma-methylmercaptobutyric acid
  • methionine
  • metionina
  • 2-ammonio-4-(methylsulfanyl)butanoate
  • methionine zwitterion
  • 1105707-18-3
  • 192948-75-7

Applications:


Amino Acids Analysis in Acid Gradient Condition



11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).



Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)

Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase.  Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Application Analytes:

Alanine
Amino Acids
Aspartic Acid
Leucine
Methionine
Valine

HPLC Separation of Amino Acids



Amino acids are building blocks for peptides and proteins. Serine is not essential to human diet and it is synthesized in human body. Methionine is not synthesized in human body and needs to be ingested. GABA is used to enhance growth of specified plants, prevent development of powdery mildew on grapes, and suppress certain other plant diseases. In humans GABA helps to maintain normal brain function. Serine, methylserine, GABA and methionine are separated on Primesep 100 column by mixed-mode mechanism. Amino acids are retained by combination of reverse phase and cation-exchange mechanisms. At lower pH carboxylic acid fragment of amino acid is suppressed and not ionized, making amino acids more basic and slightly more hydrophobic. Amount of acetonitrile, buffer concentration and buffer pH can be used to adjust retention time. Underivatized amino acids are well retained and separated with perfect peak shape and symmetry. Fast method can be used for UV, ESLD and LC/MS quantitation of serine, methylserine, GABA and methionine.



Application Analytes:

Methionine
Serine
a-Methylserine
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Serine, a-Methylserine, GABA, and Methionine Mixture


Four underivatized amino acids (serine, methylserine, GABA and methionine) were separated on a Primesep 100 reversed-phase cation-exchange mixed-mode HPLC column. Two methods, one for UV and one for ELSD/LC/MS show good separation and peak shape for underivatized amino acids. This column and general HPLC approach can be used for analysis of underivatized amino acids. Primesep 100 is designed to replace reversed-phase HPLC column in combination with ion-pairing reagents.



Application Analytes:

Methionine
Serine
a-Methylserine
gamma-Aminobutyric Acid (GABA)

HPLC Separation of Mixture of Essential Amino Acids


Application Analytes:

Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
Methionine
Phenylalanine
Tryptophan
Valine

HPLC Separation of Mixture of 12 Amino Acids


Application Analytes:

Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
Methionine
Phenylalanine
Proline
Tryptophan
Valine